一个未知的特异结合雌激素效应元件编码链的单链结合蛋白  

An Unknown Protein Binding Specifically To the Coding Strand of the Estrogen Responsive Element

作　　者：朱国璋[1] 刘峻[1] 张友端[1] 张永莲[1] 

机构地区：[1]中国科学院上海生物化学研究所分子生物学国家重点实验室

出　　处：《生物化学与生物物理学报》1996年第4期404-411,共8页

基　　金：国家自然科学基金

摘　　要：本文介绍一个不是雌激素受体（ＥＲ）却能专一地结合雌激素效应元件（ＥＲＥ）编码单链的单链结合蛋白，对此蛋白在细胞中的定位、组织中的分布、热稳定性、同二价金属离子的关系作一初步研究。发现其受雌激素负调控。此蛋白在人病理样品２９例乳癌中同ＥＲ的含量似有一定的负相关关系，讨论了上述结果的意义。An unknown protein,designated ERE-C-SSBP,which binds to the single coding strand of estrogen response element(ERE)tightly and specifically,has been discovered unexpectedly during an attempt to establish an ER-ERE gel retardation assay with a 13bp core fragment of the ERE as a probe.Some of its characteristics,such as subcellular location,tissue distribution,heat stability,relation with divalent metal ions,are reported. Interestingly,it may be down-regulated by estrogen.Its value in 29 breast carcinoma samples is to some degree in reverse correlationship with that of ER. The significance of these findings is discussed.

关 键 词：雌激素效应元件 雌激素受体 单链结合蛋白 

分 类 号：Q579.13[生物学—生物化学]