PAF对肺内小动脉平滑肌细胞TXA_2，PGI_2乃Ca^（2＋）－ATPase的影响  

EFFECT OF PLATELET ACTIVATING FACTOR ON TxA_2, PGI_2 AND Ca￣(2+)-ATPase ACTIVITY IN THE INTRA-PULMONARY ARTERY SMOOTH MUSCLE CELLS

作　　者：胡德辉[1] 马传桃[1] 

机构地区：[1]湖南医科大学心血管生理研究室,广州第一军医大学生理学教研室

出　　处：《生理学报》1996年第4期343-347,共5页Acta Physiologica Sinica

基　　金：国家自然科学基金

摘　　要：本工作采用分离培养家兔肺内小动脉平滑肌细胞（ＰＡＳＭＣｓ），观察了外源性血小板活化因子（ｐｌａｔｅｌｅｔａｃｔｉｖａｔｉｎｇｆａｃｔｏｒ，ＰＡＦ）、ＢＮ５２０２１（ＰＡＦ受体拮抗剂）、吲哚美辛、维拉帕米对ＰＡＳＭＣｓ产生血栓素Ａ_２（ＴｘＡ_２）、前列环素（ＰＧＩ_２）及对细胞膜Ｃａ^（２＋）－ＡＴＰａｓｅ活力的影响。结果表明：（１）基础状态下ＰＡＳＭＣｓ存在花生四烯酸（ＡＡ）代谢。（２）外源性ＰＡＦ通过受体后途径激活环加氧酶促进ＡＡ代谢致ＴＸＡ_２及ＰＧＩ－２增加，ＴＸＡ_２／ＰＧＩ_２比值无明显变化。（３）外源性ＰＡＦ能直接抑制Ｃａ^（２＋）－ＡＴＰａｓｅ活力。（４）维拉帕米可逆转ＰＡＦ抑制ＰＡＳＭＣｓ膜Ｃａ^（２＋）－ＡＴＰａｓｅ活力的效应。The effects of exogenous platelet activating factor (PAF), BN52021 (an antagonist of PAF receptor), indomethacin and verapamil on the production of TxA2, PGI2 and activity of Ca2+-ATPase were investigated in rabbit intra-pulmonary smooth muscle cells (PASMCs). The results were as follows: (1) Under basic condition, active AA metablism was present in PASMCs. (2) Singnificant increase of TxA2 and PGI2 but not their ratio may result from binding of PAF receptor and activiton of cyclooxgenase. (3) Activity of Ca2+-ATPase could be inhibited by exogenous PAF. (4) The inhibitory effect of PAF on PASMCs Ca2+ -ATPase could be reversed by verapamil.

关 键 词：肺动脉 平滑肌细胞 血小板活化因子 PGI2 血栓素 

分 类 号：R331.33[医药卫生—人体生理学]