转基因人参细胞中HBsAg的表达及稳定性研究  

Expression of HBsAg in Transgenic Ginseng Cells and Stability of Expressed Product

作　　者：吉海滨[1] 刘丹[1] 刘黎红[2] 李娟[1] 于海鹏[1] 富锐丽[1] 李铮[1] 盛军[1] 

机构地区：[1]长春生物制品研究所,长春130062 [2]长春职业技术学院,长春130033

出　　处：《中国生物制品学杂志》2006年第5期448-449,453,共3页Chinese Journal of Biologicals

基　　金：吉林省科技发展计划项目(No.20030405)资助

摘　　要：目的对转基因人参CS83细胞系表达HBsAg的特异性、稳定性及表达效率进行分析。方法提取CS83细胞表达蛋白,分别以Lowry法和ELISA法检测总蛋白含量和HBsAg含量,SDS-PAGE和Western blot分析表达蛋白的HBsAg特异性。采用冷冻干燥、干燥和低温处理方法检测CS83细胞中HBsAg的稳定性。结果CS83细胞表达产物中总蛋白含量为2·024mg/g,HBsAg含量为504ng/g,占总蛋白含量的0·025%。表达的目的蛋白相对分子质量约为25000,具有HBsAg特异性。经不同方法处理后的保存结果表明,冻干保存稳定性最好。结论所建立的CS83细胞系能够稳定地表达特异性HBsAg。Objective To study the specificity, stability and expression efficiency of HBsAg expressed in transgenic ginseng CS83 cells. Methods Extract expressed protein from CS83 cells and determine for total protein and HBsAg contents by Lowry method and ELISA respectively. Analyze the expression efficiency of HBsAg by SDS-PAGE and specificity by Western blot. Treat CS83 cells by lyophilization and drying,and store at different temperatures to study the stability of HBsAg. Results The total protein content of expressed product of CS83 cells was 2. 024 mg/g. The HBsAg content was 504 ng/g,accounting for 0. 025% of total protein content. The expressed product with a relative molecular weight of 25 000 showed specificity of HBsAg and good stability in a freeze-dried form. Conclusion The established transgenic ginseng CS83 cell line was suitable for stable expression of specific HBsAg.

关 键 词：人参细胞 HBSAG 稳定性 

分 类 号：R512.62[医药卫生—内科学]