猪肥胖基因ob近端启动子的生物信息学分析  

Bioinformatic analysis of swine ob proximal promoter

作　　者：李毅[1] 张嘉保[1] 赵志辉[2] 武霞[2] 张树敏[3] 

机构地区：[1]吉林大学实验动物中心,吉林长春130062 [2]吉林大学畜牧兽医学院,吉林长春130062 [3]吉林省农科院畜牧分院,吉林公主岭136100

出　　处：《广西农业生物科学》2006年第B09期31-33,共3页Journal of Guangxi Agricultural and Biological Science

基　　金：国家振兴东北老工业基地科技攻关项目(2004BA907A22);吉林省牧业科技基金项目(2005-23)

摘　　要：通过检索网上数据库资料,获得ob的转录本信息;利用F irstEF等预测程序分析并获得ob的启动子序列,并使用M atlspector程序对启动子序列的转录因子结合位点进行预测。结果表明,目前已知的ob转录本中,5′UTR区最长的序列为AF 492499(G enB ank登录号);利用F irstEF等预测程序分析成功获得了长度为529 bp的猪ob的启动子序列,M atlspector程序分析该启动子中含有24个转录因子结合位点,包括TATA盒、SP 1、C/EBP等多种顺式反应元件。通过分析获得了猪ob启动子序列及其转录调控信息。All reported transcripts of ob were collected through searching online database. The proximal promoter of ob was predicted using FirstEF online software and so on. And the binding sites of transcription factor were analysed by MatInspector. Result showed that among all the reported transcripts ofob,the 5＇UTR of AF492499 was the longest. The sequence of the proximal promoter of ob was obtainded by FirstEF. And 24 cisbinding elements were found within the proxima promoter by Matlnspector. The transcriptional information of the proximal promoter of ob was obtained.

关 键 词：OB基因 转录调控 生物信息学 

分 类 号：Q953[生物学—动物学]