猪体细胞核移植重构胚发育异常及印迹基因表达特征研究的进展  

Abnormal development and expressing characteristics of "imprinting gene" in reconstituted embryos from porcine somatic nuclear transfer

作　　者：张德福[1] 吴华莉[1] 刘东[1] 陈学进[2] 陈莹[2] 王凯[2] 

机构地区：[1]上海市农业科学院畜牧兽医研究所 [2]上海第二医科大学上海市发育生物学重点实验室,上海200025

出　　处：《广西农业生物科学》2006年第B09期145-148,共4页Journal of Guangxi Agricultural and Biological Science

基　　金：上海市科技兴农重大攻关项目(沪农科攻字[2005]第14-1号);上海市科学技术委员会项目(014909005;04DZ05611);上海市自然科学基金课题(98ZC14025);德国BMBF(CHN99/310)基金

摘　　要：由于猪的器官极可能成为人类未来器官的供应源，猪体细胞核移殖及转基因等方面的研究已成为全球的热点。近几年来，猪体细胞核移植研究取得了一定的进展，但总体来看核移植效率还很低（1％～2％），还需要不断完善核移植程序及对一些基础问题的深入研究。本文主要对猪体细胞核移植重构胚发育异常及“印迹”基因表达特征作一综述与分析，旨在为研究者提供一些有益的启示。Research in the fields of somatic cell nuclear transfer （SCNT） and transgenic cloning in pigs has become global highlight, because porcine organs would probably become the first source of donor organs for human xenotransplantation. Recently, great progress has been made in porcine SCNT. However, the efficiency of nuclear transfer remains very low （1%～2%）. It is necessary to improve the procedure of nuclear transfer and to further investigation of some basic problems. In this paper, we mainly focus on the abnormal development and expressing characteristics of ＂imprinting gene＂ in reconstituted embryos from porcine somatic nuclear transfer, including its applications and approach of increasing the efficiency of SCNT.

关 键 词：猪 核移植 印迹基因 

分 类 号：Q813.7[生物学—生物工程]