乙二胺四乙酸钠对猪卵母细胞的体外成熟和孤雌激活后早期发育的影响  

Effect of ethylenediaminetetraacetic acid on porcine oocyte maturation in vitro and subsequent early development after parthenogenetically activation

作　　者：卢晟盛[1] 魏凤[1] 刘红波[1] 房慧伶[2] 陆阳清[1] 卢克焕[1] 王爱德[2] 

机构地区：[1]广西大学广西亚热带生物资源保护利用重点实验室,广西南宁530005 [2]广西大学动物科学技术学院,广西南宁530005

出　　处：《广西农业生物科学》2006年第B09期190-190,194,共2页Journal of Guangxi Agricultural and Biological Science

基　　金：广西亚热带生物资源保护利用重点实验室-省部共建国家重点实验室培育基地开放课题(SB0505);广西科学基金项目(桂科青0640002和桂科青0447004);广西科学研究与技术开发计划项目(桂科攻0537022-01和桂科攻0330004-14);广州市华粤行仪器有限公司南宁办事处计划内横向项目

摘　　要：本研究探讨了乙二胺四乙酸钠（EDTA—Na）对猪卵母细胞的体外成熟和孤雌激活后早期发育的影响。利用屠宰场猪卵巢卵母细胞，在不添加或添加不同浓度EDTA—Na的成熟培养液中体外成熟44～48h，挑选核成熟卵母细胞进行孤雌激活，然后移到不添加或添加不同浓度EDTA—Na的胚胎培养液中进行体外培养168h。结果说明：（1）成熟液中添加适当浓度（25～100μmol／L）EDTA—Na对体外成熟猪卵母细胞孤雌激活后的早期发育具有促进作用，在EDTA—Na浓度为100μmol／L时，效果最佳；（2）胚胎培养液中添加适当浓度（25～100μmol／L）EDTA—Na，对体外成熟猪卵母细胞孤雌激活后的早期发育具有促进作用，在EDTA—Na浓度为100μmol／L时，效果最佳；（3）成熟液和胚胎培养液中同时添加100μmol／LEDTA—Na对体外成熟猪卵母细胞孤雌激活后的早期发育具有促进作用；（4）成熟液或／和胚胎培养液中添加不同浓度EDTA—Na，对体外成熟培养后的猪核成熟卵母细胞及孤雌激活后的4-细胞孤雌胚SDS—PAGE电泳的蛋白质表达图谱都无显著影响。This study was to investigate the effect of EDTA - Na on in vitro maturation of porcine oocytes and early development after parthenogenetically activation . Porcine oocytes from abattoir ovaries were matured in maturation medium whether or not containing different concentrations of EDTA-Na for 44～48 hours. Oocytes with the first polar body were parthenogenetically activated and then cultured in NCSU-23 medium whether or not containing different concentrations of EDTA-Na. The results showed： （1） Supplementation with 25～100μmol/L EDTA-Na in maturation medium was beneficial to early development of porcine parthenogenetic embryos derived from oocytes matured and artificially activated in vitro, and when EDTA-Na concentration was 100 μmol/L its effect was the best; （2） Supplementation with 25～100μmol/L EDTA-Na in embryo culture medium was beneficial to early development of porcine parthenogenetic embryos derived from oocytes matured and artificially activated in vitro, and when EDTA-Na concentration was 100 μmol/L its effect was the best; （3） Supplementation with 100μmol/L EDTA-Na in maturation and embryo culture media together was benificial to early development of porcine parthenogenetic embryos derived from oocytes matured and artificially activated in vitro; （4） Supplementation with different concentrations of EDTA-Na in maturation medium or/and embryo culture medium had no significant effect on protein expression profiles of SDS-PAGE electrophoresis of porcine oocytes matured in vitro and 4-cell parthenogenetic embryos after artificial activated.

关 键 词：猪 卵母细胞 体外成熟 孤雌激活 胚胎培养 

分 类 号：Q813[生物学—生物工程]