以分子信标为报告分子的凝血酶检测新方法  

A Novel Assay for Thrombin Detection Using Molecular Beacon as Reporter

作　　者：仲志鸿[1] 羊小海[1] 王柯敏[1] 谭蔚泓[1] 李惠敏[2] 孟祥贤[1] 郭秋平[1] 

机构地区：[1]湖南大学化学化工学院化学生物传感与计量学国家重点实验室生物医学工程中心湖南省生物纳米工程技术研究中心,中国湖南长沙410082 [2]湖南大学材料工程学院,中国湖南长沙410082

出　　处：《生命科学研究》2006年第3期206-209,共4页Life Science Research

基　　金：国家重点基础研究发展规划(2002CB513100;2004CB520804);科技部国际合作重点项目(2003DF000039);国家科技攻关计划项目(2003BA310A16);国家自然科学基金项目(20305006;20505007);湖南省科技厅重点项目(02JZY2004;0399Y1006)资助

摘　　要：以分子信标为报告分子,核酸适体为识别分子,发展了一种新的凝血酶检测方法.含有分子信标互补序列的核酸适体探针与凝血酶结合后,分子信标的荧光信号下降,从而得到凝血酶的浓度信息.该方法快速、灵敏,核酸适体探针无需荧光标记、设计简单,检测限达到0.83nmol/L.A novel assay for thrombin was developed, in which molecular beacons （MBs） and aptamers were used as reporters and recognizers, respectively. Probes composed of the cDNA sequence of MB and aptamer of thrombin were used. The fluorescence of MB decreased once aptamer bound with thrombin. The fluorescence decrease of MBs was quantitatively dependent on the concentration of thrombin. The detection limit was 0. 83 nmol/L. The design of probes was simple and no labeling was required. This assay was rapid and sensitive. It could be used for other proteins by just changing the aptamer.

关 键 词：分子信标 核酸适体 凝血酶 

分 类 号：Q343[生物学—遗传学]