山葡萄多酚对大鼠红细胞膜结构稳定性的影响  

Effects of polyphends of Vitis amurensis Rupr on stability of RBC-membrane structure in rats

作　　者：焦淑萍[1] 倪海镜[2] 杜培革[1] 

机构地区：[1]北华大学医学院生化教研室,吉林吉林132013 [2]吉林大学中日联谊医院药剂科,吉林长春130033

出　　处：《吉林大学学报（医学版）》2006年第5期829-831,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅资助课题(200302-05)

摘　　要：目的:研究山葡萄多酚(PVAR)抗衰老、抗氧化作用。方法:选用老龄(20月龄)Wistar雄性大鼠40只,采用区间随机分组方法按体重均分为4组,即老龄阴性对照组(A组),喂基础饲料;2个老龄PVAR组(B、C组),分别喂含200、400mg·kg-1·d-1PVAR的基础饲料;老龄阳性对照组(D组),喂含200mg·kg·d-1蜂花粉的基础饲料。连续饲养8周后,测定大鼠红细胞膜、血浆及肝脏MDA含量、红细胞膜巯基含量、唾液酸含量、Na+-K+-ATP酶活性和红细胞膜流动性、血浆GSH-Px活性。结果:与A组比较,B、C组和D组大鼠组织MDA含量显著降低(P<0·05或P<0·01);C组及D组红细胞膜巯基含量、唾液酸含量、红细胞膜Na+-K+-ATP酶活性及血浆GSH-Px活性显著升高(P<0·05)。B、C组与D组各项指标比较差异均无显著性(P>0·05)。结论:PVAR对老龄大鼠具有抗衰老、抗氧化作用。Objective To study the anti ageing and antioxidation of polyphends of vitis amurensis Rupr （PVAR）. Methods Forty senile male Wistar rats （20 months） were divided into 4 goups according rats＇ weights： senile negtive control group （A）, fed with basic forage; two senile PVAR groups （B and C）, fed with basic forage inchuding 200 and 400 mg ·kg^-1·d^-1 PVAR; senile positive control （D）, fed with basic forage inchuding 200 mg ·kg^-1·d^-1 melissic powder. After fed continuously for 8 weeks, the MDA contents in RBC-membrane, plasma and liver, sulfhydryl content in RBC-membrane, sialic acid content, activity of Na^＋-K^＋-ATPasc, fluidity of RBC-membrane, and activity of plasma GSH-Px in rats were detected. Results Compared with group A, the contents of MDA in groups B, C, and D decreased significantly （P〈0.05, P〈0.01）, sulfhydryl contents in RBC-membrane and activities of plasma GSH-Px in group C and D increased significantly （P〈0.05）. There were no significant differences of various indexes between group B, C and group D （P〈0.05）. Conclusion PVAR has anti-ageing and antioxidation on senile rats.

关 键 词：葡萄科 酚类/药理学 衰老 抗氧化 红细胞膜 

分 类 号：R965[医药卫生—药理学]