胞浆型磷脂酶A2基因多态性与2型糖尿病的关系  

Relationship between cytosolic phospholipase A2 gene polymorphism and type 2 diabetes mellitus

作　　者：史杰萍[1] 陶然[1] 张小娟[1] 姚燕[1] 方芳[1] 于雅琴[1] 

机构地区：[1]吉林大学公共卫生学院吉林大学基因组医学研究中心,吉林长春130021

出　　处：《吉林大学学报（医学版）》2006年第5期839-842,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30400263)

摘　　要：目的:探讨胞浆型磷脂酶A2(cPLA2)基因多态性与中国北方汉族人群2型糖尿病(T2DM)的关系。方法:采用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)方法检测T2DM患者(病例组121例)和健康对照(对照组142例)3个cPLA2基因(PLA2G4A、PLA2G4B和PLA2G4C基因)的基因型;借助UNPHASED分析平台,利用等位基因条件分析(COA)和基因型条件分析(COG)方法分析基因的联合作用与T2DM的遗传相关性。结果:病例组与对照组中3个基因位点的基因型分布均符合Hardy-Weinberg平衡定律;病例组和对照组中各基因位点的等位基因及基因型的频数分布比较差异均无显著性(P>0·05);COA分析表明BanISNP-rs1648833、BanISNP-rs1549637、rs1648833-rs1549637和BanISNP-rs1648833-rs1549637位点间的联合与T2DM不存在关联性(P>0·05);而COG分析发现,rs1648833-rs1549637联合时与T2DM相关联(P<0·05),但BanISNP-rs1648833、BanISNP-rs1549637和BanISNP-rs1648833-rs1549637位点间的联合与T2DM无关联(P>0·05)。结论:cPLA2基因多态性与中国北方汉族人群T2DM存在较弱的关联性。Objective To investigate the genetic relationship between the cytosolic phospholipase A2 （cPLA2） gene polymorphism and type 2 diabetes mellitus （T2DM） in Northern Han Chinese. Methods The method of PCR- RFLP was conducted to examine the genotype of 3 cPLA2 genes （ PLA2G4A, PLA2G4B and PLA2G4C genes） in 263 subjects, including 121 cases of T2DM and 142 controls. The conditional test was used to test the combined effect of distinct loci on the T2DM by conditioning on allele （COA） or by conditioning on genotype （COG） with UNPHASED analysis platform. Results The genotypic frequencies of the 3 genes did not deviate from Hardy- weinberg equilibrium in both case and control groups. The allelic and genotypic frequencies of the 3 genes had no remarkable difference between case and control （P〉0.05）. The COG test revealed that T2DM was associated with the rs1648833-rs1549637 combination （P〈0.05）, but the COA test did not show such an association with the combination. Neither the COA test nor the COG showed that T2DM was associated with the BanlSNP-rs1648833 and BanlSNP-rs1549637 combination. In the combination of all 3 SNPs （BanlSNP-rs1648833-rs1549637）, the COA test and the COG test didn＇t show an association （P〉0.05）. Conclusion cPLA2 gene polymorphism may be involved in the etiology of T2DM in Northern Han Chinese, although their effects are relatively small.

关 键 词：糖尿病 非胰岛素依赖型 磷脂酶A 多态现象(遗传学) 基因 

分 类 号：R587.1[医药卫生—内分泌] Q346.5[医药卫生—内科学]