肺癌和乳腺癌患者外周血中CD4^+CD25^+T细胞及foxp3 mRNA的变化特点及临床意义  

Changes of CD4^+CD25^+ T cells and expression of foxp3 mRNA in peripheral blood mononuclear cell from patients with lung cancer and breast cancer

作　　者：崔永生[1] 李欣[1] 于春雷[1] 辛精卫[1] 李一[1] 付彤[2] 

机构地区：[1]吉林大学基础医学院免疫学教研室,吉林长春130021 [2]吉林大学第一医院胸外科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2006年第5期843-846,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30571724)

摘　　要：目的:探讨肺癌、乳腺癌患者外周血中CD4+CD25+调节性T细胞水平的变化及临床意义。方法:应用流式细胞术检测9例肺癌、9例乳腺癌患者及10例非肿瘤患者外周血单个核细胞(PBMC)中CD4+CD25+T细胞占CD4+T细胞的比例,应用RT-PCR技术检测人外周血PBMCfoxp3基因的表达。结果:流式细胞术检测显示,肺癌及乳腺癌患者外周血PBMC中CD4+CD25+T细胞占CD4+T细胞的比例[(21·30±11·26)%]高于非肿瘤患者[(14·19±8·56)%](P<0·05),肺癌及乳腺癌患者外周血PBMC中foxp3mRNA的表达水平高于非肿瘤患者表达水平(P<0·05),肺癌和乳腺癌患者外周血CD4+CD25+调节性T细胞比例增加。结论:CD4+CD25+调节性T细胞在维持自身免疫稳定的同时对肿瘤免疫具有抑制作用,该细胞群比例的增加可能参与肿瘤的发生。Objective To investigate the changes of CD4^＋CD25^＋ regulator T cells in peripheral blood from the paitents with lung cancer and breast cancer. Methods Flow cytometry was used to analyze the proportion of CD4^＋CD25^＋ T cells/CD4^＋ T cells in peripheral blood mononuclear cells （PBMC） from 9 patients with lung cancer, 9 patients with breast cancer and 10 patients without cancer as controls. The expression of foxp3 mRNA in PBMCwas detected by RT-PCR. Results The value of CD4^＋CD25^＋ T cells/ CD4^＋ T cells in PBMC from patients with lung cancer and breast cancer （21.30± 11.26）% was significantly higher compared with controls （14. 19± 8.56）%. The expression of foxp3 in PBMC enhanced in patients with cancer compared with controls, there was significant difference between two groups （P〈0.05）. It revealed that the population of CD4^＋CD25^＋ regulator T cells in peripheral blood of patients with cancer was increased. Conclusion CD4^＋CD25^＋ regulator T cells may be responsible for immune suppression in the tumor immunity while maintenance of immune tolerance and the increase of CD4^＋CD25^＋ regulator T cells may be involved in tumor generation.

关 键 词：CD4^+CD25^T细胞 FOXP3 肺肿瘤 乳腺肿瘤 

分 类 号：R730.45[医药卫生—肿瘤] R730.3[医药卫生—临床医学]