少精子症、弱精子症和畸形精子症患者精子凋亡率比较  

Comparisons of apoptotic rates of sperm between patients with oligozoospermia, asthenozoospermia and teratozoospermia

作　　者：刘媛[1] 刘睿智[1] 吴迪[1] 赵竞[1] 高久春[1] 

机构地区：[1]吉林大学基础医学院细胞生物学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2006年第5期869-871,共3页Journal of Jilin University：Medicine Edition

基　　金：长春市科技局资助课题(2004066)

摘　　要：目的:比较少精子症、弱精子症和畸形精子症患者精子凋亡率。方法:应用计算机辅助精液分析(CASA)对生育组(n=14)、不育组[(n=48),少精子症(精子密度<10×106·mL-1)11例、弱精子症(a+b级精子百分率<50%)25例、畸形精子症(形态正常精子百分率<14%)12例]进行精液参数检测,瑞-姬染色检测精子凋亡率。结果:不育组及不育组中的少精子症组精子凋亡率均明显高于生育组(P<0·05);弱、畸形精子症组精子凋亡率与生育组比较差异均无显著性(P>0·05)。密度异常组(精子密度<20×106·mL-1)精子凋亡率明显高于密度正常组(精子密度≥20×106·mL-1)(P<0·05);a+b级异常组(a+b<50%)精子凋亡率与正常组比较差异无显著性(P>0·05);精子形态异常组(形态正常精子<14%)凋亡率与正常组比较差异无显著性(P>0·05)。结论:不育组精子凋亡率高于生育组,尤其是少精子症患者精子凋亡率增加更明显。Objective To compare the apoptotic rates of sperm in patients with oligozoospermia, asthenozoospermia, teratozoospermia. Methods Semen parameters were determined by computer assisted sperm analysis （CASA）. Semen samples were from 14 fertility persons and 48 infertility patients, of which 11 patients with oligozoospermia （sperm concentration〈20 ）〈 10^6·mL^-1 ）, 25 patients with asthenozoospermia （a ＋ b〈 50 % ） and 12 patients with teratozoospermia （percentage of normal morphology sperm〈 14%）. The apoptotic rates of sperm in two groups were analyzed by Wright-Giemsa staining. Results The apoptotic rates of sperm in infertility and oligozoospermia groups were significantly higher than that in fertility group （P〈0.05）. The apoptotic rates of sperm were not significantly different between asthenozoospermia and teratozoospermia groups （P〉 0.05）. The apoptotic rate of sperm in abnormal sperm concentration （sperm concentration〈 20 × 10^6·mL^-1 ） group was significantly higher than that in nomal group （P 〈0.05）. No significant difference of apoptotic rate of sperm of a＋b grade motile sperm was found between the normal group and abnormal group （P〉0.05）. The apoptotic rate of sperm was not significantly different between the normal morphology sperm group and abnormal morphology sperm group （P 〉0. 05）. Conclusion The apoptotic rate of sperm in infertility group is significantly higher than that in fertility group, specially in oligozoospermia patients.

关 键 词：不育 男(雄)性 精子 精子凋亡 

分 类 号：R698.2[医药卫生—泌尿科学]