LC/MS/MS法测定人血浆中人参皂甙-Rd浓度  

Determination of Ginsenoside Rd in Human Plasma by LC/MS/MS

作　　者：邓远辉[1] 冯怡[1] 曾星[1] 杨柳[1] 刘奕明[1] 黄羽[1] 孙静[1] 周丹[1] 梁伟雄[1] 

机构地区：[1]广东省中医院国家临床药品试验基地,广东广州510120

出　　处：《中药材》2006年第9期928-931,共4页Journal of Chinese Medicinal Materials

基　　金：国家高新技术研究发展计划(863计划)项目(2002AA2Z341B)

摘　　要：目的：建立液相色谱-质谱（LC／MS／MS）法测定人血浆中人参皂甙-Rd浓度。方法：血浆样品采用固相萃取法处理。用电喷雾离子化和正离子多离子反应监测（MRM）方式检测人参皂甙-Rd（m／z964．6→767．5；964．6→325．3），内标物：龙胆苦甙（m／z374．1→195．1；357．1→195．1）。结果：人参皂甙-Rd的最低定量限为3．00ng/ml；线性范围为3．00—5000．00ng／ml；方法回收率在81．01％-83．39％范围，日内、日间变异系数（RSD）均小于15％。结论：该法准确、灵敏、特异，适用于血浆人参皂甙-Rd浓度的测定。Objective： To develop a HPLC/MS/MS method for the determination of ginsenoside Rd in human plasma. Methods： Plasma samples were pretreated by solid phase extraction （ SPE ）. Ginsenoside Rd （ m/z 964. 6→m/z 767. 5 ） and gentiopicrin （ m/z 374. 1→195.1） was detected by the positive electrospray ionization （ESI^＋ ）-MS method under multiple reaction monitoring （MRM） mode. Results： The calibration curve of ginsenoside Rd in plasma was linear over the range of 3.00 - 5000. 00 ng/ml. The limit of quantitation was 3.00 ng/ml. The relative recovery was 81.01 -83.39%. The within-day and between-day RSDs were less than 15%. Conclusion： This method is accurate, sensitive, and specific. It is suitable for the measurement of plasma ginsenoside Rd concentration.

关 键 词：人参皂甙-Rd HPLC/MS/MS法 血药浓度 

分 类 号：R969.1[医药卫生—药理学] R977[医药卫生—药学]