3种中草药滴眼液对不同细胞株的体外细胞毒作用  

Cytotoxic Effects of Three Kinds of Eye Drops for Chinese Herbal Medicines on Various Cell Lines in Vitro

作　　者：李瑜[1] 何梅凤[1] 黄楚龙[1] 

机构地区：[1]中山大学中山眼科中心,广东广州510060

出　　处：《中药材》2006年第9期949-951,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:探讨3种中草药滴眼液对体外培养的人癌细胞和正常细胞的细胞毒作用。方法:采用噻唑蓝(MTT法)测定鱼腥草滴眼液、葛根素滴眼液和双黄连滴眼液对6种细胞株(CNE2、G lc-82、HRPE、F ibro、3T3和ECV-304)的细胞毒作用并以半数抑制浓度(IC50)进行评价。结果:鱼腥草滴眼液对人癌细胞CNE2和G lc-82的IC50分别为11.5±0.25μl/m l和24.0±0.8μl/m l;对正常细胞HRPE、F ibro、3T3和ECV-304细胞的IC50分别为18.0±3.5μl/m l、52.0±14.0μl/m l、17.5±3.5μl/m l和17.5±1.3μl/m l。葛根素滴眼液对CNE2和C lc-82细胞的IC50为9.8±2.3μl/m l和17.0±5.0μl/m l,对ECV-304细胞的IC50为10.5±0.95μl/m l,而对HRPE、F ibro和3T3的IC50均>110μl/m l。双黄连滴眼液对CNE2、G lc-82细胞的IC50为18.9±5.0μl/m l和23.9±0.6μl/m l,对HRPE细胞的IC50为113.9±25.6μl/m l,对F ibro、3T3和ECV-304的IC50均>100μl/m l。结论:鱼腥草滴眼液对人癌细胞和正常细胞均有显著的细胞毒作用。葛根素滴眼液和双黄连滴眼液对正常细胞无明显的细胞毒作用。Objective ： To study the cytotoxic effects of three kinds of eye drops on human cancer cells and normal cells. Methods ： Cytotoxic effects of Yuxingcao Eye Drop （ YXC ） , Gegensu Eye Drop （GGS） and Shuanghuanglian Eye Drop （SHL） on 6 cell lines （CNE2, Glc-82, HRPE, Fibro, 3T3 and ECV-304） were assayed using MTT method. And cytotoxic efficacy was evaluated using fifty percent of inhibitory concentration （ IC50 ）. Results ： IC50 of YXC to human cancer cells CNE2 and Glc-82 were （ 11.5 ± 0.25 ） μg/ml and （ 24. 0 ± 0. 8 ） μt/ml ; to normal cells HRPE, Fibro, 3T3 and ECV-304 were （ 18.0 ± 3.5 ） μt/ml, （52. 0 ± 14. 0） μt/ml, （ 17.5 ± 3.5 ） μt/ml and （ 17.5 ± 1.3 ） μt/ml, respectively. IC50 of GGS to CNE2, Glc-82, ECV-304 cells were （9.8 ± 2. 3 ） μt/ml, （ 17.0 ± 5.0） μt/ml and （10.5 ±0. 95）μt/ml,respectively. Under the concentration of 100 μt/ml, the average survival rates of HRPE, Fibro and 3T3 cells were 60. 0% ,87.8% and 58. 2% , respectively. IC50 of SHL to CNE2, Glc-82 and HRPE cells were （ 18.9 ±5.0） μl/ ml, （ 23.9 ± 0.6 ） μl/ml and （ 113.9 ± 25.6） μl/ml. At the concentration of 100 μl/ml, the average survival rates of Fibro, 3T3 and ECV-304 Cells were 89.6% , 77.2% and 74.7%. Conclusion ： YXC has evident cytotoxicities to human cancer cells and normal cells. While, GGS and SHL has not obvious cytotoxic effects to normal cells.

关 键 词：中草药 滴眼液 人癌细胞 正常细胞 细胞毒作用 

分 类 号：R285.5[医药卫生—中药学]