单核细胞增多性李斯特菌的脉冲场凝胶电泳分型研究  

Subtyping of Listeria monocytogenes by pulse-field gel electrophoresis

作　　者：曾海燕[1] 孙箴[2] 

机构地区：[1]青海大学,青海西宁810016 [2]浙江大学医学院分子与细胞学研究中心,浙江杭州310031

出　　处：《青海大学学报（自然科学版）》2006年第5期51-55,共5页Journal of Qinghai University(Natural Science)

摘　　要：用限制性内切酶SmaI和ApaI分别对21株单核细胞增多性李斯特菌菌株基因组DNA进行酶切,用脉冲场凝胶电泳(PFGE)方法进行遗传多样性分析。结果表明:3株临床分离株(00181、00174和0194)分别存在于亚型A、B、D中;16个单核细胞增多性李斯特菌奶相关和环境分离株主要存在于4个亚型中,其中2个亚型(C、D)均为奶相关分离株(包括原料奶、均质奶(储奶罐)和成品奶分离株),而加工厂地面污水分离株为独立的一个亚型(A)。21 L. motwcytogenes strains were digested by Sma I and Apa I,then typed by pulse field gel electmphoresis. All tested L. monocytogenes strains were grouped into five elusters. Clusters A, D and E were related to milk including raw milk, homogenized milk and pasteurized milk, while the strains isolated from sewages and other processing environments were in an independent cluster. These results indicate that L. monocytogenes strains contaminating the pasteurized milk products most probably came from raw milk but not from processing environments.

关 键 词：单核细胞增多性李斯特菌 脉冲场凝胶电泳 分型 

分 类 号：Q781[生物学—分子生物学]