镓转铁蛋白与HL60细胞的结合  

Binding of Gallium-Containing Transferrin to HL60 Cells

作　　者：李英奇[1] 刘斌[1] 赵春贵[1] 杨斌盛[1] 

机构地区：[1]山西大学分子科学研究所化学生物学与分子工程教育部重点实验室,太原030006

出　　处：《化学学报》2006年第18期1933-1936,共4页Acta Chimica Sinica

基　　金：国家自然科学基金(Nos.20071022;20371031);山西省自然科学青年基金(No.20051006);山西大学校青年基金(No.2006008)资助项目.

摘　　要：用125I示踪法测定了不同形式的镓转铁蛋白(GaC-Tf,Ga2-Tf,GaC-Tf-FeN)与HL60细胞的结合作用.结果表明0℃、pH=7.4时GaC-Tf,Ga2-Tf和GaC-Tf-FeN均与细胞膜结合,表观结合常数分别为(1.2±0.14)×108,(1.1±0.19)×108和(1.8±0.42)×108L/mol;同时测定了125I标记的脱铁转铁蛋白(apoTf)与HL60细胞的表观结合常数为(0.97±0.20)×108L/mol;HL60细胞的平均受体数为(4.8±0.11)×105.通过体外研究表明,不同形式的镓转铁蛋白与受体的结合能力次序为apoTf≈GaC-Tf≈Ga2-Tf<GaC-Tf-FeN.The interaction of ^12SI-labelled various species of transferrin including GaC-Tf, Ga2-Tf and GaC-Tf-FeN with HL60 cells was investigated. The results showed that the proteins bound to membrane of cells at pH=7.4 and 0 ℃. The association constants were （1.2±14）×10^8, （1.1±0.19）× 10^8 and （1.8± 0.42） × 10^8 L/mol for GaC-Tf, Ga2-Tf and GaC-Tf-FeN, respectively. At the same time, the association constant for ^125I-labelled apotransferrin was （0.97±0.20）× 10^8 L/mol. There were about （4.8±0.11）× 10^5 transferrin receptors per cell surface. In vitro studies indicated that complexes of Ga and transferrin bind to transferrin receptor with the order of apoTf≈GaC-Tf≈Ga2-Tf〈Gac-Tf-FeN in terms of their association constants.

关 键 词：转铁蛋白 镓(Ⅲ) ^125I示踪法 HL60细胞 

分 类 号：Q51[生物学—生物化学]