电子探针X线微区分析1－烯丙基氯－3引起神经组织内Na、K、Ca等元素的改变  

AC-INDUCED PERCENTAGE CHANGES OF Na, K,Ca, etc.ELEMENTS IN NERVE TISSUE DETERMINED BY EPMA

作　　者：谢克勤[1] 高树君[1] 张磊[1] 孙克任[1] 

机构地区：[1]山东医科大学毒理学研究室

出　　处：《山东医科大学学报》1996年第3期205-208,共4页Acta Academiae Medicinae Shandong

基　　金：国家自然科学基金

摘　　要：用ＥＰＭＡ技术测定了１－烯丙基氯－３染毒后鸡胚脑神经细胞、大鼠坐骨神经轴浆和脑干内Ｎａ、Ｋ、Ｃａ、Ｍｇ、Ｃｌ、Ｐ元素的百分含量变化。结果显示，在鸡胚脑神经细胞、大鼠坐骨神经轴浆和脑干内Ｎａ、Ｃａ明显增加，Ｋ则明显减少（Ｐ＜０．０１）。在鸡胚脑神经细胞内Ｃｌ明显增加（Ｐ＜０．０１），而在大鼠坐骨神经轴浆和脑干内无明显变化。在大鼠坐骨神经轴浆和脑干内Ｍｇ明显增加，而在鸡胚脑神经细胞内明显减少（Ｐ＜０．０５，Ｐ＜０．０１）。在鸡胚脑神经细胞内和大鼠坐骨神经轴浆内Ｐ明显减少（Ｐ＜０．０５），而在大鼠脑干内减少不明显。１－烯丙基氯－３引起神经组织内Ｎａ、Ｋ、Ｃａ、Ｍｇ、Ｃｌ、Ｐ元素稳态失调。Using electron probe X-ray microanalysis(EPMA),the ACinduced percetage changes of Na, K, Ca, Mg, P elements in chicken embryon brain cells, rat sciatic nerve axons and stems were investigated. The results showed that Na and Ca elements were increased,however,K element decreased markedly in chicken embryon brain cells, rat sciatic nerve axons and stems(P<0. 01). Cl in chicken embryon brain cell was increased significantly(P<0. 01) ,but in rat sciatic nerve axons and stems its percentages had no significant differences compared with control,Mg in rat sciatic nerve axons and stems increased markedly but decreased in chicken embryon brain cells(P<0. 05, P< 0. 01 ). P decreased in chicken embryon brain cells and rat sciatic nerve axons(P<0. 05),however,no significant differences in rat stems. The results reveal that AC may result in unhomeostasis of Na. K, Ca, Cl, P in nerve tissue and the AC induced toxic peripheral neuropathy might be associated with those element unhomeostasis in nerve tissue.

关 键 词：烯丙基氯 钠 钾 钙 神经组织 毒理学 电子探针 

分 类 号：R994.1[医药卫生—毒理学] R338[医药卫生—药学]