脱乙酰几丁质酶降解产物的纸层析分析  

Paper Chromatography of Chitosanase's Hydrolysates

作　　者：蒋桂芳[1] 任文彬[1] 张世清[1] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南省热带农业有害生物检测监控重点实验室,海南儋州571737

出　　处：《华南热带农业大学学报》2006年第3期28-31,共4页Journal of South China University of Tropical Agriculture

基　　金：科技部"国家科技基础条件平台工作("编号:2004DKA30560)

摘　　要：对本实验室筛选保存的几株产脱乙酰几丁质酶菌株的产酶条件,如pH值、氮源和金属离子进行初步研究,结果表明,其酶作用最适pH范围为5.0～6.0,氮源及Cu2+、Co2+和Mn2+对脱乙酰几丁质酶有不同程度的促进作用,Zn2+对脱乙酰几丁质酶有较强的抑制作用。用纸层析测定这些菌株在不同产酶条件下发酵产物中壳寡糖的含量和成分,根据产物情况推测酶作用方式,表明不同条件下得到壳寡糖有所变化。This paper analyzes the effects of some chitoanase-producing microorganisms on chitosanases and enzymatic hydrolystas by applying paper chromatography. Chitosanase＇s optimal pH was 5.0-6.0, N source, metalion Cu^2＋, Co^2＋ and Mn^2＋ can promote chitosanase＇s activity, but Zn^2＋ exerts negative effect, restraining the chitosanase＇s activity. The zymolytic production is not the same in different conditions. Chitosan oligosaccharide is mostly got from chitosan endonuclease. E7 was selected on the basis of incubation condition and paper chromatography.

关 键 词：脱乙酰几丁质酶 壳寡糖 培养条件 纸层析 

分 类 号：Q55[生物学—生物化学] O636.1[理学—高分子化学]