鲫鱼视网膜谷氨酸受体和GABA受体在爪蟾卵母细胞中的表达  

STUDIES ON GLUTAMATE AND GABA RECEPTOR EXPRESSED IN AMPHIBIAN OOCYTES AFTER INJECTION OF CARP RETINA mRNA

作　　者：包永德[1] 朱辉[1] 朱幸[1] 

机构地区：[1]中国科学院上海生理研究所

出　　处：《生理学报》1996年第4期401-404,共4页Acta Physiologica Sinica

基　　金：国家自然科学基金;中国科学院上海生命科学联合开放实验室和中国科学院上海生命研究中心资助

摘　　要：我们以两栖类卵母细胞为功能表达系统，通过注射鲫鱼（Ｃａｒａｓｓｉｕｓｃａｒａｓｓｉｕｓ）视网膜ｍＲＮＡ，利用电压箝及药物灌流手段，系统地研究了鲫鱼视网膜内氨基酸受体的类型和特征，结果如下：（１）Ｇｌｕ受体：ＫＡ可以诱发明显的去极化电流，而且Ｄｉａｚｏｘｉｄｅ能增强ＫＡ诱导的反应，这提示鲫鱼视网膜内某些Ｃｌｕ受体是ＡＭＰＡ选择性亚型（ＡＭＰＡ－ｐｒｅｆｅｒｒｉｎｇｓｕｂｔｙｐｅ）。（２）ＣＡＢＡ受体：ＧＡＢＡ能诱发一个快速、光滑的内向电流，绝大部分对ＧＡＢＡ的反应可被ｂｉｃｕｃｕｌｌｉｎｅ所压抑，而ＧＡＢＡ＿Ｂ受体的激动剂ｂａｃｌｏｆｅｎ则无任何作用，这提示，鲫鱼视网膜内大部分是ＧＡＢＡ＿Ａ受体。Using voltage clamp and perfusion technique, the types and characteristics of amino acid receptors of carp (Carassius carassius ) retinas were studied by expressed respouse of amphibian oocytes previous injected with the retinal mRNA. The results were as follows: (1 ) Gin receptor: Diazoxide could potentiated KA induced depolarization,suggesting that some of the retinal Gin receptors may be AMPA-preferring subtype.(2) GABA receptor: Bicuculine could greatly suppress the GABA induced inward rapid current, while GABAB agonist baclofen was without effect. This suggests that the most of retinal GABA receptors may be GABAA type.

关 键 词：鲫鱼 神网膜 爪蟾 卵母细胞 谷氨酸 Γ氨基丁酸 

分 类 号：Q959.468[生物学—动物学] Q959.530.5