豚鼠腹腔神经节迟慢兴奋性突触后电位与5－HT和P物质的关系  

THE RELATIONSHIP OF LATE SLOW EXCITATORY POTENTIAL WITH 5-HYDROXYTRYPTAMINE AND SUBSTANCE P IN THE GUINEA-PIG CELIAC GANGLION

作　　者：裴文芬[1] 蔡圣荣[1] 祝延[1] 孔德虎[1] 黄振信 马如纯 

机构地区：[1]安徽中医学院生理学教研室,安徽医科大学生理学教研室

出　　处：《生理学报》1996年第3期263-268,共6页Acta Physiologica Sinica

基　　金：国家自然科学基金

摘　　要：应用细胞内记录，研究了豚鼠腹腔神经节（ＣＧ）细胞非胆碱能迟慢兴奋性突触后电位（ＬＳ－ＥＰＳＰ）与５－羟色胺（５－ＨＴ）及Ｐ物质（ＳＰ）的相互关系。当重复电刺激内脏大神经（ＳＮ）时，有７８．２％的细胞（１６１／２０６）在动作电位发放后出现ＬＳ－ＥＰＳＰ，灌注或压力注射５－ＨＴ或ＳＰ，分别在６８．５％的细胞（１０２／１４９）及５２．１％的细胞（９８／１８８）上引起５－ＨＴ或ＳＰ去极化反应，两者差异非常显著（Ｐ＜０．０１）；大部分具有ＬＳ－ＥＰＳＰ的细胞对５－ＨＴ（７３／８８，８３．０％）或ＳＰ（６８／１１４５９．６％）敏感，而不出现ＬＳ－ＥＰＳＰ的细胞仅有少数对５－ＨＴ（１０／２６，３８．５％）或ＳＰ（１１／３６，３０．６％）敏感，两类细胞的差异非常显著（５－ＨＴ：Ｐ＜０．０００１，ＳＰ：Ｐ＜０．０１）。上述结果支持５－ＨＴ与ＳＰ均作为递质参与ＬＳ－ＥＰＳＰ形成的观点。此外，在１３３个细胞上同时检测了５－ＨＴ与ＳＰ的作用，其中有６６个细胞（４９．６％）对５－ＨＴ及ＳＰ均敏感，提示在ＣＧ细胞，５－ＨＴ与ＳＰ之间可能存在某种机能联系。The work was carried out to investigate the relationship of non-cholinergic late slow excitutory potential (LS-EPSP) with 5-hydroxytryptamine (5-HT) and substance P (SP) in the neurons of the guinea pig celiac ganglion (CG) using intracellular electrodes in vitro. During repetitive stimulation of the splanchnic nerve (SN), LS-EPSP following a series of action potentials could be recorded in 161 out of 206 neurons (78. 2% ); Application of 5-HT and SP by super fusion or pressure ejection induced 5-HT depolarization in 102 out of 149 neurons (68. 5 % ) and SP depolarization in 98 out of 188 neurons (52. 1 % ), respectively 3 Most neurons, from which LS-EPSP could be recorded during stimulation of SN, were sensitive to 5-HT (73/88, 83. 0 % ) and SP (68/114, 59. 7 % ). However, only a small number of neurons not showing LS-EPSP during stimulation of SN were sensitive to 5-HT (10/ 26, 38. 5%, P< 0. 0001 ) and SP (11 /36, 30. 6 %, P<0. 01 ). The results support the viewpoint that both 5-HT and SP are involved in the formation of LS-EPSP as transmitters; In addition,both effects of 5-HT and SP were examined in 133 neurons. There were 66 of these neurons (49. 6 %) to be sensitive to both 5-HT and SP, suggesting that there may be some functional relations between 5-HT and SP in the neurons of guinea pig CG.

关 键 词：迟慢兴奋性 突触后电位 P物质 神经节 血清素 

分 类 号：R338.13[医药卫生—人体生理学]