xylE基因用于监测根瘤菌在土壤中存活的研究  

Use of a xylE marker gene to monitor the survival of rhizobia populations in soil

作　　者：崔明学[1] 张成刚[1] 靳素英[1] 张晓东 李明祺[1] 李宁[1] 蔺继尚 

机构地区：[1]中国科学院沈阳应用生态研究所

出　　处：《应用生态学报》1996年第3期287-292,共6页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金

摘　　要：通过细菌接合将质粒ｐＬＶ１０１６（含ｘｖｌＥ基因）转移至ＲｈｉｚｏｂｉｕｍｆｒｅｄｉｉＱＢ１１３０和Ｒｈｉｚｏｂｉｕｍｌｅｇｕｍｉｎｏｓａｒｕｍｂｖ．ｖｉｃｉａｅＢ４０，ｘｙｌＥ基因在根瘤菌内表达活性较高．质粒ｐＬＶ１０１６携带的ＸｙｌＥ基因用于监测根瘤菌在灭菌和未灭菌土壤中的存活，结果表明，在灭菌土壤中含质粒与不含质粒菌株存活菌数量之间无显著差异（Ｐ＜０．０５），大接种量有利于细菌的生长与繁殖，Ｂ４０（ｐＬＶ１０１６）质粒丢失比例很低．以低于１０６ＣＦＵ·ｇ－１干土浓度接种时，ＱＢ１１３０（ｐＬＶ１０１６）质粒丢失率随接种浓度的降低而增大．未灭菌土壤中生物因素抑制了释放菌株的生长，大接种量有利于细菌存活．以低浓度（１０６ＣＦＵ·ｇ－１干土）接种时，ＱＢ１１３０（ｐＬＶ１０１６）质粒丢失比例高于Ｂ４０（ｐＬＶ１０１６）．XylE gene containing plasmid pLV1016 is introduced into Rhizobium fredii QB1130 and Rhizobium leguminosarum bv.viciae B40 by bacterial conjugation,and the expression level of xylE gene in rhizobia is quite high.The use of xyLE gene carried by plasmid pLV1016 to monitor the survival of rhizobia in sterile and non-sterile soils shows that in sterile soil,there is no significant difference(P<0.05) between the survival of strains containing and free of plasmid. Large inoculum quantity is.advantage to the growth and reproduction of bacteria,and the loss rate of plasmid B40(pLV1016):very low.When the inoculation is made with a concentration lower than 106 CFU·g-1 dry soil,the loss rate of plasmid QB1130(pLV1016) is increased with decreasing inoculation concentration.In non-sterile soil,biotic factors can inhibit the growth of released strains,and large inoculum quantity is advantage to the survival of bacteria. When the inoculation is made with a concentration lower than 106 CFU·g-1 dry soil, the loss rate of plasmid QB1130(pLV1016)is higher than that of plasmid B40 (PLV1016).

关 键 词：xylE基因 监测标记 根瘤菌 存活 土壤 

分 类 号：S154.381[农业科学—土壤学]