百萨偃麦草高分子量麦谷蛋白亚基的鉴定及其染色体定位  

Identification and Chromosomal Location of a High Molecular Weight Glutenin Subunit from Thinopyrum Bessarabicum

作　　者：李辉[1] 李义文[2] 陈豫[2] 贾旭[2] 

机构地区：[1]河北省农林科学院粮油作物研究所河北省作物遗传育种实验室,河北石家庄050031 [2]中国科学院遗传与发育生物学研究所国家植物细胞与染色体工程重点实验室,北京100101

出　　处：《河北农业科学》2006年第3期1-5,共5页Journal of Hebei Agricultural Sciences

基　　金：河北省博士基金项目(06547005D-1)

摘　　要：经SDS-PAGE电泳和W estern杂交,在百萨偃麦草(Thinopyrum bessarabicum)中鉴定出1个高分子量麦谷蛋白亚基,其电泳迁移率大于“中国春”小麦的1Dy12亚基,本研究命名为1Ebx亚基,其编码基因命名为Glu-1Ebx。对由百萨偃麦草衍生的双二倍体及其1套二体附加系进行SDS-PAGE分析,只有百萨偃麦草、双二倍体和1Eb附加系表达1Ebx亚基,小麦亲本及其它附加系均不表达此亚基。用1对HMW-GS通用引物对上述材料进行扩增时,只有在百萨偃麦草、双二倍体和1Eb染色体附加系的基因组DNA中扩增出分子量大小一致的DNA片段,与SDS-PAGE的定位结果一致。表明1Ebx亚基是由百萨偃麦草1Eb染色体上的HMW-GS基因所编码。A high molecular weight glutenin subunit （HMW-GS） which has a faster electrophoretic mobility than that of 1Dy12 subunit from the bread wheat Chinese Spring, was identified from Thinopyrum bessarabicum by SDS-PAGE and Western Blotting, designated as 1E^bx in this study. In order to determine the precise location of 1E^bx subunit, SDS-PAGE analysis was carried out in amphidiploid and additional lines deduced from Thinopyrum bessarabicum. Results of SDS-PAGE revealed that 1E^bX subunit was specially expressed in Thinopyrum bessarabicum, amphidiploid and 1E^b additional lines, other than wheat parent lines and other additional lines. Consistence with the location identified by SDS-PAGE, a band with the similar molecular weight was also amplified only in Thinopyrum bessarabicum, amphidiploids and 1E^b additional lines using a pair of degenerate primers of HMW-GS. These results showed that the 1E^bx subunit was encoded by a HMW-GS gene, Glu-1E^bx, on chromosome 1E^b of Thinopyrum bessarabicum.

关 键 词：百萨偃麦草 高分子量麦谷蛋白亚基 染色体 定位 

分 类 号：S512.9[农业科学—作物学]