马粪海胆的染色体制备及组型分析  

The chromosome preparation and karyotype analysis of sea urchin Hemicentrotus pulcherrimus

作　　者：常亚青[1] 曹洁[1] 张彦娇[1] 丁君[1] 

机构地区：[1]大连水产学院农业部海洋水产增养殖学与生物技术重点开放实验室,辽宁大连116023

出　　处：《大连水产学院学报》2006年第3期247-250,共4页Journal of Dalian Fisheries University

基　　金：国家"863"项目资助(2002AA628170)

摘　　要：采用改进的染色体制备方法,分别对马粪海胆Hem icentrotus pulcherrimus受精卵的二细胞期、八细胞期和囊胚早期的细胞进行染色体制备,结果显示,于囊胚早期取样的马粪海胆,其滴片效果最好。通过组型分析确定马粪海胆的染色体数目为2n=42,组型公式为2n=42=20m+20 sm+2 s。The chromosomes in different developmental stages such as two -celled stage, four -celled stage and early blastula were prepared from sea urchin Hemicentrotus pulcherrimus by an improved method. It was found that good chromosome occurred at early blastula stage by dripping. The results showed that the chromosomal number were 2n =42, and the karyotype 2n =42 =20m ＋20sm ＋2st.

关 键 词：马粪海胆 染色体 组型 

分 类 号：Q343.2[生物学—遗传学]