正交试验法优选金银花中绿原酸水提工艺的研究  

Study on the optimum water extraction process for chlorogenic acid from Lonicera japonica Thunb.by orthogonal design

作　　者：宋德花[1] 李继昌[1] 梁运霞[2] 刘广文[2] 杨长志 刘永 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030 [2]黑龙江畜牧兽医职业学院 [3]黑龙江出入境检验检疫局

出　　处：《中兽医医药杂志》2006年第5期5-7,共3页Journal of Traditional Chinese Veterinary Medicine

摘　　要：目的:优选金银花中绿原酸水提工艺。方法:采用正交试验法,以测定金银花中绿原酸提取率作为考核指标。结果:金银花中绿原酸水提工艺的最佳提取条件为不浸泡,加水(15+12)倍量,煎煮(1+1)h。结论:该试验绿原酸提取率高,稳定性好。Objective： To study the optimum water extraction process for chlorogenic acid from Lonicera japonica Thunb.. Methods： Orthogonal design was used to determine the optimum extraction process with the extraction rate of chlorogenic acid as markers. optimum tra process for chlorogenic acid from Lonicera japonice Thunb.was that the amount of water was 15＋12 times and decocting time was 1＋1 hous without soaking.Conclusion：The extraction process for chlorogenic acid shows high extraction rate and good atability.

关 键 词：金银花 绿原酸提取率 正交试验 高效液相色谱法 

分 类 号：S853.74[农业科学—临床兽医学]