胃泌素受体放射配基结合分析法的建立  

Establishment of radioligand binding assay of gastrin receptors

作　　者：黄广建[1] 邓守真[1] 乐竹琴[1] 张延龄[1] 王德昭[1] 余枫[1] 

机构地区：[1]上海医科大学附属华山医院

出　　处：《核技术》1996年第11期653-656,共4页Nuclear Techniques

摘　　要：建立了胃泌素受体（ＧＲ）的放射配基结合分析法，测定３４例胃癌及邻近正常粘膜胃泌素受体含量及亲和力Ｋｄ。发现五肽胃泌素（Ｇｓ－５）及［Ｌｅｕ１５］Ｇｓ－１７均能作为非标记配基，Ｇｓ－５的抑制效果更好。醋酸纤维混合膜（Φ０．４５μｍ）分离结合及游离部分１２５［Ｌｅｕ１５］Ｇｓ－１７效果好。３４例胃癌及其邻近正常粘膜表达ＧＲ是一致的。Radioligand binding assay of gastrin receptor(GR) was established to determine GR content and Kd of gastric cancer and mucosa in specimens of 34 patients. Both of Gs-5 and [len15]Gs-17, as unlabelled ligand, can inhibit the binding of 125I-(leu15)Gs-17to the membrane, and the inhibiting effect of Gs-5 is slightly stronger than that of (len15)Gs-17.Mixed cellulose acetate filters have better effect to separate bound 125I (leu15)Gs-17 from free 125I- (len15)Gs-17 than glass fibre filters (Hongguan 999999).The GR of gastric cancer correlates with that of mucosa in the same region of 34patients.

关 键 词：胃泌素受体 放射配基 结合分析 胃癌 

分 类 号：R817.45[医药卫生—影像医学与核医学]