氚标记胆红素的研制及应用  

Investigation on 3H-labelled bilirubin for Study of blood-brain barrier

作　　者：曹蓉珍[1] 董墨[1] 张雨龙[1] 周瑞菊[1] 

机构地区：[1]中国科学院上海原子核研究所

出　　处：《核技术》1996年第12期754-758,共5页Nuclear Techniques

基　　金：上海市科委基金

摘　　要：由胆绿素经钠硼氚还原生成氚标记胆红素，而胆绿素是由胆红素经ＤＤＱ脱氢氧化形成，钠硼氚是在镍催化及高温下由销硼氢与氚气交换得到。氚标记胆红素比活度为３０６ＴＢｑ／ｍｏｌ，放化纯度经高效液相色谱及纸层析鉴定均大于９５％，用氚标记胆红素进行开放血脑屏障的动物实验获得了满意的结果。ynthesis of 3H-labelled bilirubin is described.3H-bilirubin is prepared by the reduction of biliverdin using sodium boro-[3H]-hydride in methanol solvent.But biliverdin is synthesized through dehydrogenation of bilirubin with 2, 3-dichloro-5,6-dicyanobenzoquinone(DDQ)in dimethyl sulphoxide and sodium boro-[3H]-hydride is produced by exchange of sodium boro-hydride with tritium gas using nickel catalyst at high temperature.The specific activity of obtained 3H-bilirubin is 306GBq/mmol,while the radiochemical purity is over 95% by HPLC and paper chromatography.The permeatied profile of 3H-labelled bilirubin in rat brain has been satisfyingly obtained in animal experiments.

关 键 词：氚标记 胆红素 胆绿素 钠硼氚 

分 类 号：Q486[生物学—生理学]