蝴蝶兰未受精胚珠离体培养的研究  

In Vitro Culture of Unfertilized Ovules in Phalaenopsis

作　　者：伍成厚[1] 潘一山[1] 罗开梅[1] 叶秀粦[2] 梁承邺[2] 

机构地区：[1]漳州师范学院生物系,福建漳州363000 [2]中国科学院华南植物园,广东广州510650

出　　处：《园艺学报》2006年第4期891-894,共4页Acta Horticulturae Sinica

基　　金：国家自然科学基金资助项目(30170061);广东省重大科技专项资助项目(2003A2010401);福建省青年科技人才创新项目资金(2004J052)

摘　　要：以蝴蝶兰大孢子母细胞时期的胚珠为外植体进行了离体培养的研究。结果表明,在VW+BA0·2mg·L-1+NAA0·2mg·L-1+蔗糖2·0%+植物凝胶0·3%中蝴蝶兰的大孢子母细胞可以发育至二核胚囊;在VW+BA0·2mg·L-1+NAA0·2mg·L-1+蔗糖2·0%+植物凝胶0·3%+椰子汁10%中大孢子母细胞可以发育至四核胚囊或形成胚性细胞团,这些胚性细胞团有可能发育成单倍体植株。The effects of medium composition on the in vitro culture of unfertilized ovules of a Phalaenopsis hybrid were studied. The results showed that the megasporocytes of the hybrid were capable of undergoing meiosis to form hi-nucleate embryo sacs in vitro by ovule cultures on the medium of VW ＋ BA O. 2 mg· L^-1 ＋ NAA0.2 mg· L^-1 ＋ sucrose 2.0% ＋phytage10.3%. On the medium ofVW ＋ BA0.2 mg· L^-1 ＋ NAA 0. 2 mg · L^-1 ＋ sucrose 2.0% ＋ phytagel 0. 3% ＋ coconut milk 10%, the megasporocytes would develop into 4-nucleate embryo sacs or embryogenic cell masses. These embryogenic cell masses may develop into haploid plants.

关 键 词：蝴蝶兰 胚珠 胚性细胞团 组织培养 

分 类 号：S682.31[农业科学—观赏园艺]