鸡传染性喉气管炎病毒gB基因重组DNA疫苗的构建与免疫试验  

Construction and Immune Effect of the Recombinant DNA Vaccine Expressing ILTV Major Antigen Epitope gene

作　　者：姬向波[1] 刘文波[1] 魏建超[1] 牛明福[1] 周斌[1] 曹瑞兵[1] 陈溥言[1] 

机构地区：[1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏南京210095

出　　处：《中国病毒学》2006年第5期481-484,共4页Virologica Sinica

摘　　要：本研究构建了编码ILTV主要抗原gB基因的重组DNA疫苗pcDNA-gB,质粒转染293-T细胞的间接免疫表明其表达的蛋白具有免疫反应性。为测定该DNA疫苗的免疫效果,将其与本室保存的重组鸡痘病毒rFPV-gB-gD-IgY分别以单独和混合的方式给4周龄非免疫鸡进行免疫,然后测定ILTV特异性抗体和T淋巴细胞增殖反应。结果表明这2种基因工程疫苗均能诱导鸡产生特异性的体液免疫及细胞免疫应答。其中以pcDNA-gB/rFPV-gB-gD-IgY联合免疫组的效果最好,其诱导的抗体水平已接近于常规弱毒疫苗,而细胞免疫水平则比后者高得多。上述研究结果为ILTV新型疫苗的研究奠定了基础。A Plasmid DNA vaccine pcDNA-gB expressing gB protein of infectious laryngotracheitis virus（ILTV） Yantai strain was constructed. The expression of viral protein from constructed DNA vaccine was confirmed by an in vitro transcription/translation system and transtection in 293-T cells. To evaluate the prophylactic efficacy of the DNA vaccine, 4-week-old chickens were inoculated with pcDNA-gB plasmid and rFPV- gB-gD-IgY, respectively followed by a booster of rFPV-gB-gD-IgY and pcDNA-gB. Anti-ILTV antibodies and T cell proliferation were detected. The results indicated that both vaccines could elicit specific humeral immune and cellular immune responses. Compared with attenuated vaccines, rFPV-gB-gD-IgY/pcDNA-gB elicited not only similar antibody level of ILTV but stronger T cell proliferation. The study lays foundation on further investigation for the new type of vaccines against ILTV.

关 键 词：传染性喉气管炎 DNA疫苗 糖蛋白B 重组鸡痘病毒 免疫效果 

分 类 号：S852.4[农业科学—基础兽医学] Q78[农业科学—兽医学]