猪繁殖与呼吸综合征病毒S1株基因组序列测定和分析  

Genomic Sequencing of S1 Strain of a Porcine Reproductive and Respiratory Syndrome Virus Isolate

作　　者：李玉峰[1] 姜平[1] 许家荣[1] 蒋文明[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095

出　　处：《中国病毒学》2006年第5期490-493,共4页Virologica Sinica

基　　金：国家自然科学基金(30270990);教育部优秀青年教师资助计划(20030307012);新世纪优秀人才资助计划(NCET-04-0502);教育部重点基金项目(104101)

摘　　要：应用RT-PCR方法分段扩增出PRRSV上海分离株S1毒株的4条基因大片段,扩增后的产物分别克隆于pCR-XL-TOPO载体鉴定后测序,同时应用RACE方法对S1毒株的3′和5′基因末端进行了成功的扩增并克隆于pMD-18T载体进行测序,按顺序将这些序列进行拼接得到PRRSVS1株全基因组cDNA序列。测序结果表明PRRSVS1株基因组全长15441bp,包含9个开放式阅读框,5′UTR含有189nt,3′端UTR含有181nt,其中包含30ntPoly(A)。基因组序列分析结果显示该病毒与ATCCVR-2332和BJ-4分离株的核苷酸同源性分别99.5%和99.6%。与另一国内分离株CH-1a的核苷酸同源性为90.8%。Four gene fragments of S 1 strain of porcine reproductive and respiratory syndrome virus were amplified by RT-PCR and cloned into the pCR-XL-TOPO vector for sequencing. Authentic 5＇ and 3＇ terminal fragments of S1 strain were obtained by 5＇-Full RACE Core Set and 3＇ -Full RACE Core Set and cloned into the pMD-18T vector and sequenced. The complete nucleotide sequence of S 1 strain genome was obtained by sequential splicing of each fragment. The results showed that the genome of S1 strain was 15441 bp in length, containing nine open reading frames （ORFs）, with 189 nt in the 5＇ UTR and 181 nt in the 3＇ UTR, including 30 nt poly （A） tail. The genome of PRRSV S1 strain showed 99.5%, 99.6% and 90.8% of nucleotide identity with ATCC VR-2332, BJ-4 and CH-1a, respectively.

关 键 词：PRRSV 基因组 序列测定和分析 

分 类 号：S852.65[农业科学—基础兽医学]