免疫受损大鼠感染铜绿假单胞菌时细胞间黏附分子1及基质金属蛋白酶2、9的变化  

Lung immunohistochemical study on the changes of ICAM-1,MMP-2 and MMP-9 in an immunocompromised rat model infected with Pseudomonas aeruginosa pneumonia

作　　者：李倬哲[1] 瞿介明[1] 何礼贤[1] 

机构地区：[1]复旦大学附属中山医院肺科,上海200032

出　　处：《中华传染病杂志》2006年第4期225-229,共5页Chinese Journal of Infectious Diseases

基　　金：上海市卫生系统"百人计划"项目(98BR030)

摘　　要：目的了解免疫受损大鼠铜绿假单胞菌肺部感染前后肺组织中细胞间黏附分子(ICAM)-1、基质金属蛋白酶(MMP)-2和MMP-9的改变及其与肺部炎症反应和肺损伤的关系。方法建立免疫受损大鼠铜绿假单胞菌肺部感染的模型,观察其肺部炎症反应,测定湿/干比和支气管肺泡灌洗液中总蛋白(TP)了解肺损伤情况,对其肺组织分别行ICAM-1、MMP-2和MMP-9免疫组织化学研究。结果1．铜绿假单胞菌肺部感染后,两组大鼠肺泡上皮细胞中ICAM-1染色强度较未感染时增加,接种后6h、9h、24h明显高于接种前(P＜0．05);2．两组大鼠在铜绿假单胞菌肺部感染后,肺组织中MMP-2染色增强,于接种后6h或9h达高峰,并持续至24h。两组大鼠感染前后MMP-2在各组织结构中的积分差异无统计学意义。MMP-9在感染前后的改变与MMP-2相仿; 3．两组大鼠肺组织中ICAM-1积分与中性粒细胞半定量计数具有相关性(对照组r_s=0．641,P= 0．001,免疫受损组r_s=0．668,P＜0．001);4．两组大鼠肺组织中MMP-2、MMP-9染色积分与PMN半定量计数呈正相关(P＜0．01);5．免疫受损组大鼠支气管上皮细胞中MMP-9染色强度积分与TP具相关性(r_s=0．484,P＜0．05),肺泡上皮细胞、小血管内皮细胞及内皮下组织中MMP-2染色强度与TP皆具相关性(r_s分别为0．457、0．492、0．429,P＜0．05)。结论免疫受损大鼠肺部铜绿假单胞菌感染后,肺组织中ICAM-1、MMP-2、MMP-9免疫组织化学显示染色增强,于感染后6～9h达高峰,与免疫受损大鼠肺组织中明显的中性粒细胞浸润及严重的肺损伤有关。Objective To observe the immunohistochemical changes of ICAM-1, MMP-2 and MMP-9 in the lungs of irnrnunocornprornised rats with Pseudornonas aeruginosa pneumonia and their relationships with lung inflammation. Methods After the establishment of pseudornonas aeruginosa pneumonia infected irnrnunoeornprornised rat mode, the pathological changes of lungs were observed, lung wet/dry ratios and total protein concentration in bronchial alveolar lavage fluid were tested, and irnunnohistoehernical study of ICAM-1, MMP-2 and MMP9 in lung tissue were performed. Results 1. The staining intensity of ICAM-1 in alveolar epithelial cells turned stronger in rats with pulmonary infection than those without of both groups （P 〈 0.05） ; 2. The staining intensity of MMP-2 in lung tissue was stronger in rats with pulmonary infection than those without infection in both groups, and reached peak at 6～9 h after inoculation. Immunohistochemical changes of MMP-9 exhibited a similar pattern; 4. Immunohistochemical changes of ICAM-1, MMP-2 and MMP-9 showed some correlation with numbers of polymorphonuclears in lung tissue （P 〈 0.05 ） ; 5. A correlation between the stai ning intensity of MMP 9 in bronchial epithelial cells and total protein concentrations were observed （rs = 0. 484, P 〈 0.05）, similar association were found between the staining intensity of MMP 2 in alveolar epithelial cells, endothelium of arterioles and venules and tissues beneath endothelium and total protein concentrations in bronchial alveolar lavage fluid （rs were 0. 457, 0. 492 and 0. 429, respectively, P 〈 0. 05）. Conclusion In immunocompromised rats, the staining intensity of ICAM-1, MMP-2 and MMP-9 in lung tissue of those with pseudomonas aeruginosa pneumonia were stronger than those without infection, and the changes were demonstrated some correlation with the levels of polymorphonuclears infiltration or severity of lung injury.

关 键 词：免疫抑制法 假单胞菌 铜绿 免疫组织化学 血管细胞黏附分子-1 明胶酶A 明胶酶B 

分 类 号：R516[医药卫生—内科学]