甲氨蝶呤磁靶向脂质体的制备及初步评价  

Preparation of Stable Magnetoliposomes with High Encapsulation Efficiency of Methotrexate

作　　者：朱林[1] 王璐璐[1] 倪坤仪[1] 童昕[2] 戴亮[1] 屠洁 

机构地区：[1]中国药科大学,南京210009 [2]南京市口腔医院,南京210009 [3]深圳市制药厂,广东深圳518029

出　　处：《中国药学杂志》2006年第17期1324-1327,共4页Chinese Pharmaceutical Journal

基　　金：南京市医学科技发展项目(ZKX0308)

摘　　要：目的通过对甲氨蝶呤磁靶向脂质体的处方及制备工艺研究,研制高包封率和稳定的脂质体。方法采用逆向蒸发法制备甲氨蝶呤磁靶向脂质体,以正交实验优化处方;测定了脂质体中药物的包封率和脂质体的粒径;分离并测定了磁性脂质体中包裹的铁磁性物质的含量;初步考察了脂质体的稳定性。结果甲氨蝶呤磁靶向脂质体的包封率大于60%,平均粒径为627.8nm,多分散系数为0.131,每毫升脂质体乳浊液包裹铁磁性物质为116.1μg,具有良好的稳定性。结论所有的实验结果提示,该制备工艺和处方可以制备具有高包封率及稳定性的甲氨蝶呤磁靶向脂质体。OBJECTIVE To get an optimized formulation of the stable manetohposomes with high encapsulation efficiency of methotrexate. The methotrexate magnetoliposomes were prepared by reverse evaporating method. The formulation was optimized by orthogonal design. The encapsulation efficiency, the particle size and the content of encapsulated Fe2O3 were determined. RESULTS The encapsulation efficiency of the methotrexate reached 60%, and the particle size of the liposome was 627.8 nm with the polydispersity index of 0.131. The concentration of the encapsulated Fe2O3 was 116.1 μg. CONCLUSION The results suggest that this method can be used to prepare a stable magnetoliposomes with high encapsulation efficiency of methotrexate.

关 键 词：甲氨蝶呤 磁靶向脂质体 逆向蒸发法 包封率 稳定性 

分 类 号：R979.1[医药卫生—药品] TQ460[医药卫生—药学]