在海藻酸钠凝胶上诱导骨髓间充质干细胞分化为成骨细胞  

Induction of Bone Mesenchymal Stem Cells to Form Osteoblast on Alginate Gels

作　　者：王嫣[1] 陈小菊[1] 王兰[2] 左国伟[1] 寇小琴[1] 谭启华[1] 周兰[1] 陈文直[1] 

机构地区：[1]重庆医科大学医学超声工程研究所,重庆400016 [2]英国剑桥大学生物工程学研究所

出　　处：《中国生物工程杂志》2006年第9期38-42,共5页China Biotechnology

基　　金：国家自然科学基金资助项目(30470885)

摘　　要：通过在海藻酸钠凝胶上诱导bMSCs向成骨细胞分化,探讨其对骨髓间充质干细胞(bonemesenchymalstemcells,bMSCs)的生物学效应。采用MTT、甲苯胺蓝染色、vonKossa染色和RT-PCR分别检测细胞的增殖、生长形态、诱导后细胞的钙化结节和成骨相关基因的表达。实验组bMSCs生长状况良好、细胞增殖迅速,与对照组的增殖无差异;bMSCs成集落样生长明显,集落中央细胞重叠生长形成钙化结节;培养至12天,实验组和对照组的成骨相关基因,包括碱性磷酸酶、I型胶原和骨钙素,均为阳性表达,但实验组的表达量高于对照组。海藻酸钠凝胶能够促进bMSCs向成骨细胞的分化,是良好的骨组织工程支架材料。Bio-material is material, alginate gels, on bone a key factor in bone tissue engineering. The biological effects of a new biomesenchymal stem cells （bMSCs） were investigated, bMSCs were induced to form osteoblasts on alginate gels. Cell growth, appearance and calcification were detected by MTT, toluidine blue stain and yon Kossa stain, respectively. Osteoblast related genes were examined by RT-PCR. It is found that bMSCs on the alginate gels （experimental group） grew well and formed obvious colony. The genes related with osteoblast （ alkaline phosphatase, collagen the control group （bMSCs on tissue culture were higher than those in the control group. into osteoblasts. This suggests that alginate Key words Mesenchymal stem cell I and osteocalcin） were positive both in the experimental group and plastic）, moreover, the expression levels in the experimental group The results show that alginate gels may act as a favourable gels can promote bMSCs to differentiate scaffold in bone tissue engineering.

关 键 词：间充质干细胞 海藻酸 组织工程 

分 类 号：R318[医药卫生—生物医学工程]