糖尿病大鼠视网膜细胞凋亡和钠、钾离子含量的变化(英文)  

Apoptosis and the changes of Na^+ and K^+ contents in retina of rats with diabetes mellitus

作　　者：唐柳苹[1] 何剑峰[1] 杜娟[1] 李向荣[1] 黄艳霞[1] 

机构地区：[1]柳州市人民医院眼科,广西壮族自治区柳州市545001

出　　处：《中国临床康复》2006年第40期176-177,共2页Chinese Journal of Clinical Rehabilitation

基　　金：广西自然科学基金资助(桂科自0007036)~~

摘　　要：背景:糖尿病视网膜病变是糖尿病病程中常见的慢性血管性并发症,其发生过程中与细胞凋亡及其离子水平变化的关系尚需验证。目的:观察糖尿病大鼠视网膜组织中细胞凋亡量和钠、钾离子含量的变化。设计:随机对照实验。单位:柳州市人民医院眼科。材料:实验在柳州市人民医院眼科完成。选用成年纯系雄性Wistar大鼠40只,鼠龄20个月,体质量320～350g,由中山大学医学院动物中心提供。方法:将40只Wistar大鼠随机分成对照组和实验组,每组20只。实验组用链脲佐菌素诱发糖尿病。并在糖尿病发病后分别于第4、6、12、16周检测视网膜细胞凋亡和视网膜组织中钠、钾离子含量的变化;对照组单纯用等量柠檬酸盐缓冲液注射。对2组空腹血糖、糖化血红蛋白、钠、钾离子及糖尿病病程进行相关性分析。主要观察指标:①钠、钾离子含量变化。②空腹血糖浓度、糖化血红蛋白水平。③细胞凋亡量变化。④各指标间相关性分析。结果:实验组视网膜细胞溶解液中在发病后4周可测到细胞凋亡,且随糖尿病病程的延长凋亡程度逐渐加重;视网膜组织内钠离子浓度增加、钾离子浓度明显减少(P<0.01或0.05)。实验组视网膜细胞凋亡的程度与空腹血糖浓度、糖化血红蛋白水平、视网膜内钠离子含量及糖尿病病程呈正相关(r分别为7.584,7.844,7.369,6.246,P均<0.01);而与视网膜内钾离子含量呈高度负相关(r=-7.658,P<0.01)结论:糖尿病大鼠视网膜中存在细胞凋亡和钠、钾离子含量的异常,这些变化是糖尿病视网膜病变的病理基础之一。BACKGROUND： Diabetic retinopathy is a commonly chronic-vascular complication in a course of diabetes mellitus （DM）, and its relation with apoptosis and changes of ion level needs to be proved. OBJECTIVE： To observe apoptosis and changes of Na^＋ and K^＋ contents in retinal tissue of DM rats. DESIGN： Randomized controlled study. SETTING： Department ofOphthalmology, Liuzhou Municipal People＇s Hospital. MATERIALS： The experiment was completed at the Department of Ophthalmology, Liuzhou Municipal People＇s Hospital. A total of forty adult male Wistar rats, aged 20 months, weighing 320-350 g, were provided by Animal Center of Medical College Affiliated to Sun Yat-sen University. METHODS： Forth Wistar rats were randomly divided into control group and experimental group with 20 in each group. Streptozotocin was used to induce DM in the experimental group. Apoptosis and changes of Na^＋ and K^＋ contents in retinal tissue were measured on the 4^th, 6^th, 12^th and 16^th weeks after DM onset. Rats in the control group were injected only with the same volume of citrate buffer solution. Then, correlations on the aspect of fasting blood glucose （FBG）, hemoglobin glycosylation （HbA1c）, Na^＋ and K^＋ contents and DM course were analyzed between the two groups. MAIN OUTCOME MEASURES： （1） Na^＋ and K^＋ contents;（2） FBG concentra tion and HbA1c level; （3） changes of apeptosis; （4） correlations among markers. RESULTS： Apoptosis could be detected in retinal tissue in the experimental group at 4 weeks after DM onset, and with course elongating, level of apoptosis was aggravated gradually. Na^＋ concentration was increased in retinal tissue, but K^＋ concentration was decreased （P 〈 0.01 or 0.05）. Levels of apoptosis in retinal tissue in the experimental group were positive correlation with FBG concentration, HbAlc level, Na^＋ content and DM course （r=7.584, 7.844, 7.369, 6.246; P 〈 0.01）; however, they were negative correlation with K^＋ content

关 键 词：糖尿病 视网膜 细胞凋亡 钠 钾 疾病模型 动物 

分 类 号：R587.1[医药卫生—内分泌]