噪音避水应激中心理因素对大鼠免疫功能的影响  

Effect of psychological factors on immune functions of rats in water avoidance stress

作　　者：施炳龙[1] 胡家露[1] 潘伯荣[2] 仇军文[1] 季万胜[1] 吴开春[1] 樊代明[1] 

机构地区：[1]第四军医大学西京医院消化病研究所,陕西省西安市710032 [2]第四军医大学西京医院肿瘤中心,陕西省西安市710032

出　　处：《世界华人消化杂志》2006年第23期2344-2348,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨噪音联合避水应激中的心理因素对大鼠免疫功能的影响．方法:成年(?)SD大鼠32只,随机分为实验A, B,C组和对照组．A组大鼠接受避水应激．B组接受噪音应激,C组接受噪音避水复合应激．应激结束后,检测所有大鼠肠系膜淋巴细胞增殖反应和外周血NK细胞杀伤功能．结果:以B淋巴细胞为主的淋巴细胞增殖实验及以T淋巴细胞为主的淋巴细胞增殖实验结果均显示,避水组与对照组淋巴细胞刺激指数无明显差异(P>0．05);噪声组的增殖指数低于对照组(LPS:0．71±0．11 vs 1．00±0．00,P<0．05; PHA:0．68±0．08vs1．00±0．00,P<0．05):复合应激组刺激指数明显低于对照组(LPS:0．4±0．05vs1．00±0．00,P<0．01:PHA:0．46±0．06vs1．00±0．00,P<0．01),且与噪声组也存在差异(LPS:0．4±0．05 vs 0．71±0．11,P<0．05; PHA:0．4±0．05 vs 0．68±0．08,P<0．05)．避水组与对照组的NK细胞杀伤活性无明显差异(P>0．051;而噪声组NK细胞杀伤活性低于对照组(1／10:24．6±11．6 vs 39．5±13．1,P<0．05; 1／20:21．8±9．6 vs 34．6±10．4．P<0．05;1／40: 17．8±7．9vs30．2±10．6,P<0．05);复合刺激组NK细胞杀伤活性明显低于对照组(1／10:17．6±8．3vs39．5±13．1,P<0．01;1／20:14．9±5．8 vs34．6±10．4．P<0．01:1／40:10．2±4．3 vs 30．2±10．6,P<0．01),且与噪声组也存在差异(1／10: 17．6±8．3vs24．6±11．6．P<0．05;1／20:14．9±5．8 vs 21．8±9．6．P<0．05;1／40:10．2±4．3vs 17．8±7．9．P<0．05)．结论:应激导致大鼠免疫功能下降,噪音联合避水应激中的心理因素对大鼠免疫系统抑制起一种潜在的作用．AIM： To observe the effect of psychophysical factors on the immune function of rats in water avoidance stress. METHODS： Thirty-two adult male SD rats were randomly divided into four groups （A, B, C and D）. The rats in group A, B and C received the stress induced by water avoidance, noise, noise plus water avoidance, respectively. The rats in group D served as controls. After stress, the proliferative ability of the mesentery lymphocytes and the cytotoxicity of natural killer （NK） cells were analyzed. RESULTS： The proliferative abilities of B and T dominant lymphocytes were not significantly different between group A and D （P 〉 0.05）, while they were markedly decreased in group B （LPS： 0.71 ± 0.11 vs 1.00 ± 0.00, P 〈 0.05; PHA： 0.68 ± 0.08 vs 1.00 ± 0.00, P 〈 0.05） and C （LPS： 0.4 ± 0.05 vs 1.00 ± 0.00, P 〈 0.01; PHA： 0.46 ± 0.06 vs 1.00 ± 0.00, P 〈 0.01）. The minimal proliferatative ability was found in group C, and significant difference was found between group B and C （LPS： 0.4 ± 0.05 vs 0.71 ± 0.11, P 〈 0.05; PHA： 0.4 ± 0.05 vs 0.68 ± 0.08, P 〈 0.05）. The cytotoxicity of NK cells in group A and D showed no significant difference （P 〉 0.05）, but it decreased significantly in group B （1/10：24.6 ± 11.6 vs 39.5 ±13.1, P 〈 0.05; 1/20：21.8 ± 9.6 vs 34.6 ± 10.4, P 〈 0.05; 1/40：17.8 ± 7.9 vs 30.2 ± 10.6, P 〈 0.05）. Again, the minimal cytotoxicity of NK cells was found in group C （1/10：17.6 ± 8.3 vs 39.5 ± 13.1, P 〈 0.01; 1/20：14.9 ± 5.8 vs 34.6 ±10.4, P 〈 0.01; 1/40：10.2 ± 4.3 vs 30.2 ± 10.6, P 〈 0.01）, and significant difference was found between group B and C （1/10： 17.6 ± 8.3 vs 24.6 ± 11.6, P 〈 0.05; 1/20：14.9 ±5.8 vs 21.8 ± 9.6, P 〈 0.05; 1/40：10.2 ±4.3 vs 17.8 ± 7.9, P 〈 0.05）. CONCLUSION： Under stress, the immune functions of rats are undermined, and the psycho- physical factors have potential inhibitory effects in noise plus water avoidance stress.

关 键 词：避水 噪声 应激 心理因素 免疫功能 

分 类 号：R395[哲学宗教—心理学]