拟青霉代谢物提取物对小鼠体内单胺氧化酶和慢性应激大鼠海马单胺类神经递质的影响  

Effect of bioactive compounds from paecilomyces tenuipes on monoaminoxidase activity in mice and levels of monoamine neurotransmitter in hippocampus of rats with chronic unpredictable stress

作　　者：阚红卫[1] 郑丽芳[1] 尹艳艳[1] 明亮[1] 

机构地区：[1]安徽医科大学药理学教研室,安徽省合肥市230032

出　　处：《中国临床康复》2006年第38期100-102,共3页Chinese Journal of Clinical Rehabilitation

基　　金：安徽省科技厅重点科研项目(04023048);安徽省教育厅重点科研项目(2004KJ194ZD)~~

摘　　要：目的:观察一种拟青霉代谢物提取物对小鼠体内组织单胺氧化酶活性和慢性应激大鼠海马单胺类神经递质的影响。方法:实验于2004-08在安徽医科大学药理学教研室进行。①将180只小鼠随机分为吗氯贝胺组和拟青霉代谢物提取物组,雌雄各半。吗氯贝胺组一次性灌胃吗氯贝胺40mg/kg,拟青霉代谢物提取物组均灌胃拟青霉代谢物提取物100mg/kg。按照取材时间分为0,0.25,0.5,1,2,4,8,l6,24h9个时间点,每个时间点10只大鼠。采用紫外分光光度计在每个时间点测定小鼠脑、肝组织单胺氧化酶活性。②选用成年雄性SD大鼠48只,随机分为6组:对照组、模型组、吗氯贝胺组、拟青霉代谢物提取物80,40,20mg/kg组,每组8只。采用慢性应激法造成大鼠抑郁模型,用药组及模型组大鼠共接受20d各种不同的应激(包括电击足底、4℃冷水刺激5min、45℃热刺激5min、高速水平摇晃10min、夹尾60s、禁水24h、禁食48h、悬吊5min和昼夜颠倒,平均每种刺激各2次)。刺激同时用药组灌胃给药,1次/d,共22d。模型组及对照组灌胃蒸馏水,吗氯贝胺组灌胃剂量为40mg/kg、单胺氧化酶剂量组灌胃剂量分别为20,40,80mg/kg。采用高效液相-电化学法检测应激大鼠海马肾上腺素、5-羟色胺、多巴胺及其代谢产物5-羟基吲哚乙酸、3,4-二羟基苯乙酸的含量。结果:所用实验动物全部进入结果分析。①拟青霉代谢物提取物100mg/kg1次性给药对小鼠脑组织单胺氧化酶A具有一定的选择性抑制作用,拟青霉代谢物提取物给药后4h,对小鼠脑组织单胺氧化酶A的抑制率达到66.7%。②与对照组比较,模型组大鼠海马肾上腺素、多巴胺、5-羟色胺的含量降低,3,4-二羟基苯乙酸升高,5-羟基吲哚乙酸无明显改变,多巴胺/3,4-二羟基苯乙酸,5-羟色胺/5-羟基吲哚乙酸的比值降低;与模型组比较,拟青霉代谢物提取物80,40mg/kg组肾上腺素、多巴胺、5-羟色胺的含量�AIM： To study the effects of bioactive compounds from paecilomyces tenuipes （BCPT） on monoaminoxidase （Mao） activity in brain and liver organs of mice and the levels of monoamine neurotransmitters in hippocampus of chronic unpredictable stress rats. METHODS： The experiment was conducted in the Department of Pharmacology of Anhui Medical University in August 2004. ①180 mice were randomly divided into moclobemide group and BCPT group with half females and half males. Mice in the modobemide group were intragastrically administrated with moclobemide 40 mg/kg, and mice in the BCPT group were given intragastric administration of BCPT 100 mg/kg. The ultraviolet spectrophotometer was adopted to measure the Mao activity in brain and liver tissues of mice respectively at 0, 0.25, 0.5, 1, 2, 4, 8, 16 and 24 hours with 10 mice tested in each time-point.②Forty-eight adult SD rats were selected and randomly divided into six groups： control group, model group, moclobemide group, BCPT 80,40,20 mg/kg groups with 8 rats in each group. The rat models of depression were established by chronic stress, and rats in the model group and administration group were given different stress for 20 days （including sole-shocking, stimulating at 4 ℃ in water for 5 minutes, 5- minute thermal stimulus, 10-minute weaving at high speed, 60-second tail- clamping, 24-hour water-prohibition, 48-hour fasting, 5-minute suspending and reversed time feeling for twice each）. Gastric perfusion was given to rats of the administration group at the same time of stimulating once a day for 22 days. Rats in the model group and control group were given intragastric administration of distilled water, while rats in the moclobemide group received intragastric administration at 40 mg/kg. Rats in BCPT large dose group were treated by intragastric administration respectively at 20,40, 80 mg/kg. The levels of adnephrin, 5-hydroxyptamine （HT）, dopamine and its metabolic products 5-hydroxyindoleacetic acid, 3, 4-dihydroxybenzene in hippocampus o

关 键 词：拟青霉 应激 单胺氧化酶 生物胺神经递质类 

分 类 号：R345[医药卫生—基础医学]