贻贝水溶性多糖MP-Ⅰ的分离纯化及体外抗肿瘤活性研究  

Isolation and purification of water-soluble polysaccharide MP-Ⅰ from Mytilus coruscus and study on its in vitro anti-tumor activity

作　　者：徐红丽[1] 郭婷婷[2] 郭一峰[1] 张建鹏[1] 冯伟华[1] 焦炳华[1] 

机构地区：[1]第二军医大学基础医学部生物化学与分子生物学教研室,上海200433 [2]上海水产大学生命学院中心实验室,上海200090

出　　处：《第二军医大学学报》2006年第9期998-1001,共4页Academic Journal of Second Military Medical University

基　　金：国家高技术研究发展计划/863计划(2002AA2Z3325);浙江省科技计划项目(2005ZJKJJH2202)~~

摘　　要：目的:分离纯化贻贝多糖MP-Ⅰ,并进行体外抗肿瘤活性的研究。方法:采用热水提取,Sevage法除蛋白分离得粗多糖,DEAE-Sepharose、Sepharose CL-6B柱层析纯化的方法从东海厚壳贻贝中得到贻贝多糖纯品MP-Ⅰ。用GC及TLC法进行单糖组分的分析,用MTT法进行体外抗肿瘤活性的研究。结果:得到贻贝多糖纯品MP-Ⅰ,多糖的得率为2.14%。单糖组分分析显示MP-Ⅰ主要由葡萄糖组成,MP-Ⅰ(0.5,0.1,0.02 mg/ml)对体外HO-8910、MCF-7、K562、SMMC-7721肿瘤细胞均有不同程度抑制作用(P<0.01),其中高浓度组对HO-8910、MCF-7肿瘤细胞的抑制率分别达到30.55%和36.38%。结论:贻贝多糖MP-Ⅰ主要由葡萄糖组成,对HO-8910、MCF-7、K562、SMMC-7721肿瘤细胞有体外抑制作用。Objective：To separate and purify polysaccharide MP- I from Mytilus coruscus and to investigate its in vitro anti-tumor activity. Methods： The crude polysaccharide was extracted from Mytilus coruscus with hot water and the protein was removed using Sevage method. The polysaccharide MP- I was purified using DEAE-Sepharose and Sepharose CL-6B column chromatography. Monosaccharides analysis was carried out using thin-layer chromatography and gas chromatography. The in vitro anti-tumor activity of MP- I was assessed using MTT method. Results： The yield of MP- I was 2.14%. MP- I is mainly constituted by glucose. MP- I （at 0.5, 0. 1, 0.02 mg/ml） had different degrees of inhibitory effects on HO-8910, MCF-7, K562, and SMMC-7721 tumor cells in vitro（P〈0. 01）. The inhibitory rates of MP-I at 0. 5 mg/ml against HO-8910 and MCF-7 cells were 30.55 % and 36.38 %, respectively. Conclusion： Polysaccharide MP- I is mainly constituted by glucose and it has inhibitory effect on tumor cell lines HO-8910, MCF-7, K562, and SMMC-7721 in vitro.

关 键 词：贻贝 多糖类 分离和提纯 抗肿瘤药 

分 类 号：R282.77[医药卫生—中药学]