周围动脉闭塞性病变治疗前后脊髓及背根神经节P物质的变化(英文)  

Changes in substance P in spinal cord and dorsal root ganglion before and after peripheral arterial occlusive disease

作　　者：王军 张伟 易军[3] 宁莫凡[3] 王警卫 常江 

机构地区：[1]武装警察部队陕西总队医院麻醉科 [2]解放军62160部队卫生所 [3]解放军第四军医大学西京医院普外三科

出　　处：《中国临床康复》2006年第38期186-188,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:周围动脉闭塞性病变是一种常见的周围动脉疾病,发病机制及病变过程尚不完全明确,其临床症状多较重,且治疗效果不佳。目的:观察肢体负压对周围动脉闭塞性病变犬中枢神经脊髓及背根神经节中P物质免疫反应阳性神经纤维的影响。设计:随机对照的实验研究。单位:武警陕西总队医院麻醉科和解放军第四军医大学西京医院普外三科。对象:实验于2003-04/2004-05在解放军第四军医大学西京医院动物实验室完成。选用健康杂种清洁级犬17只,体质量12～18kg,动物编号后,按随机数字表法分为3组,治疗组10只,非治疗组5只,正常对照组2只。方法:将治疗组动物制作左后肢缺血模型,在造模后14d开始行患肢负压治疗,1次/d,15min/次,负压治疗10d后行动物灌注。分别切取L1～L5的脊髓及背根神经节,行免疫组织化学染色,检测P物质免疫反应阳性神经纤维;非治疗组除不进行患肢负压治疗外,处理、检查均同治疗组。正常对照组不行缺血模型制作及负压治疗,仅行免疫组织化学染色检测。主要观察指标:各组犬脊髓及背根神经节的感觉神经纤维中P物质的变化。结果:非治疗组脊髓及背根神经节中,患侧P物质免疫反应阳性纤维(平均灰度值分别为:70.20±3.70,67.00±3.30)较正常对照组(20.00±2.80,35.50±6.40)染色明显加深,而治疗组(48.50±6.60,51.70±4.00)较非治疗组染色变浅,但仍较正常对照组加深(P均<0.01)。结论:周围动脉闭塞性疾病发病后,脊髓及背根神经节中的P物质免疫反应阳性神经纤维数量明显增多,可能是机体的一种自身保护机制。肢体负压疗法可减少肢体动脉闭塞性病变伤害性刺激的传入,缓解肢体疼痛。BACKGROUND： Peripheral arterial occlusive disease is a kind of commonly seen peripheral arterial disease. Its pathogenesy and process of pathological change are still unclear. Most of its clinical symptoms are very severe and therapeutic effect is not very good. OBJECTIVE： To observe the effect of limb negative pressure on sub- stance P （SP）-positive-immunoreactive nerve fibers in the spinal cord and dorsal root ganglion of dogs with peripheral arterial occlusive disease. DESIGN： A randomized and controlled experimental trial. SETTING： Department of Anesthesiology, Shaanxi Provincial Corps Hospital, Chinese People＇s Armed Police Forces; Third Department of General Surgery, Xijing Hospital, Fourth Military Medical University of Chinese PLA. MATERIALS： The experiment was carried out in the animal laboratory, Xijing Hospital, Fourth Military Medical University of Chinese PLA between April 2003 and May 2004. Totally 17 healthy hybrid dogs, of clean degree, weighing 12 to 18 kg, were enrolled in the experiment. After being numbered, they were randomly divided into 3 groups： treated group （n=10）, non-treated group （n=5） and normal control group （n=2）. METHODS： Left hindlimb ischemia models were created on the animals in the treated group. Fourteen days after modeling, the affected limbs were treated by negative pressure, once a day, 15 minutes once. Perfusion was given 10 days after negative pressure treatment. Segment L1-L5 spinal cord and dorsal root ganglion were dissected respectively, lmmunohistochemical staining was conducted to detect SP-positiveimmunoreactive nerve fibers; In the non-treated group, treatment and detection of affected limbs were performed as those in the treated group, but negative pressure treatment was omitted. Ischemia model preparation and negative pressure treatment were not conducted in the normal control group, and only immunohistochemical staining was given. MAIN OUTCOME MEASURES： Changes in SP of sensory nerve fiber of spinal cord and dorsal

关 键 词：P物质 动脉闭塞性病变 下身负压 脊髓 神经节 脊 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]