益气化瘀利水方干预兔膝骨关节炎软骨中胰岛素样生长因子1的变化  

Influence of Yiqihuayulishuifang on insulin-like growth factor-1 in cartilage of osteoarthritis rabbits

作　　者：高翔[1] 吴弢[1] 王拥军[2] 胡志俊[2] 施杞[2] 瞿佶[1] 李蠡[1] 滕松茂[1] 

机构地区：[1]华东医院伤外科,上海市200040 [2]上海中医药大学脊柱病研究所,上海市200437

出　　处：《中国临床康复》2006年第39期95-97,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察益气化瘀利水方对实验性兔骨关节炎软骨中胰岛素样生长因子1的影响。方法:实验于2003-07/12在上海中医药大学脊柱病研究所完成。取雄性6月龄新西兰大白兔20只,随机分为正常组、模型组、益气化瘀利水方组和氨糖美辛组,每组5只。按Colombo法建立家兔膝骨关节炎模型,术后第5～8周给药。益气化瘀利水方组给予益气化瘀利水方(由生黄芪、汉防己、左秦艽、地鳖虫、制苍术、川牛膝、全当归、仙灵脾、生米仁等组成,自制制剂)0.09g/(kg·d),氨糖美辛组给予氨糖美辛(非甾体类消炎镇痛药物,批号030401)0.09g/(kg·d),均与兔饲料混合均匀,制成定量颗粒饲料,确保其当天吃完。正常组及模型组均未给药。各组于术后第9周取右胫骨平台关节软骨,采用免疫组化方法进行染色,光镜下观察,并利用图像分析测定其胰岛素样生长因子1表达的积分吸光度。结果:20只新西兰大白兔均进入结果分析。①正常组软骨表面光滑,软骨细胞为圆形呈散在、无序排列。模型组软骨表面广泛碎裂、剥脱,可见软骨细胞簇集。益气化瘀利水方组软骨表面可见小的碎裂,软骨细胞呈散在排列。氨糖美辛组软骨表面粗糙不平,软骨细胞呈散在排列,可见细胞集簇现象。正常组、益气化瘀利水方组、氨糖美辛组软骨细胞及间质中胰岛素样生长因子1染色呈淡黄色。模型组在簇集的软骨细胞的胞浆和细胞核内胰岛素样生长因子1染色呈黄褐色。②各组关节软骨切片胰岛素样生长因子1染色强度的积分吸光度图像分析:模型组明显高于正常组(17.08±2.27,4.75±0.88,P<0.01);益气化瘀利水方组和氨糖美辛组比较,差异无显著性(10.43±2.37,9.27±2.44,P>0.05);益气化瘀利水方组和氨糖美辛组明显低于模型组(F=29.71,P<0.01)。结论:益气化瘀利水方可抑制骨质增生,其作用可能是通过调控胰岛素样生长因子1而达到的。AIM： To observe the effect of Yiqihuayulishuifang on insulin-like growth factor-1 （IGF-1） in cartilage of osteoarthritis （OA） rabbits. METHODS： The experiment was carried out in the Institute of Spine, Shanghai University of Traditional Chinese Medicine from July to December 2003. Twenty male New Zealand rabbits of 6 months old were randomized into normal group, model group, Chinese herb group and non-steroidal anti-inflammatory drugs （NSAIDs） group, with 5 rabbits in each. OA models were made according to Colombo＇s method. From the 5^th week to 8^th week post-operation, Chinese herb group and NSAIDs group were treated daily with 0.09 g/kg Yiqihuayulishuifang （included mongolian milkvetch root, fourstamen stephania root, left largeleaf gentian root, ground beetle, atractylodes rhizome, medicinal ctathula root, Chinese angelica, shorthorned epimedium herb and jobstears seed） and 0.09 g/kg glucosamine indomethacin entric-coated tablets （lot number： 030401） respectively, and the animals of normal rabbits and model rabbits were not administrated. All the animals were sacrificed at the ninth week to fetch right tibia articular cartilage, The immunohistochemistry staining was used to detect the expressions of IGF-1 in cartilage. Articular cartilages were observed with microscopy and image analysis method was used to measure the expression intensity of IGF-1. RESULTS： All 20 rabbits were involved in the final analysis.①In normal group, the cartilage was smooth in surface and the round cartilage cells arranged disorderly, In model group, the surface of cartilage presented extensive fragmentations and denudations in clusters. In Chinese herb group, the cartilage surface showed small fragmentations and cartilage cells scattered. In NSAIDs group, the cartilage surface was uneven and cartilage cells scattered in clusters. IGF-1 was stained as light yellow in cartilage cells and mesenchymal cells of normal group, Chinese herb group and NSAIDs group, while IGF-1 expressed chiefly in membrane

关 键 词：骨关节炎/中药疗法 软骨 胰岛素样生长因子Ⅰ 

分 类 号：R684.3[医药卫生—骨科学]