冬虫夏草提取液对糖尿病小鼠肝线粒体氧化损伤的保护效应  

Prosecutable function of Cordyceps sinensis extracts for hepatic mitochondrial oxidative injuries in diabetic mice

作　　者：张蕾[1] 陈顺志[1] 刘树森[2] 

机构地区：[1]锦州医学院生化教研室,辽宁省锦州市121000 [2]中国科学院动物研究所生物膜与膜生物国家重点实验室,北京市100080

出　　处：《中国临床康复》2006年第39期132-134,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:从线粒体呼吸链角度分析中药冬虫夏草提取液对糖尿病引起的氧化损伤的保护作用。方法:实验于1998-03/2000-07在中国科学院动物研究所生物膜与膜生物国家重点实验室完成。雄性昆明小白鼠32只,随机分为4组,每组8只。正常对照组不加任何处理因素,虫草组、维生素E组和糖尿病组小鼠用四氧嘧啶建立实验性糖尿病小鼠模型,分别给予口服虫草提取液(1mL/只)和皮下注射维生素E溶液(4mg/kg),糖尿病组不用任何保护作用药物。利用比色法从线粒体水平测定抗氧化酶活性,用铁氰化钾脉冲法测定线粒体呼吸活链Ⅱ+Ⅲ电子传递与质子泵出偶联状况(H+/2e)。结果:32只小鼠均进入结果分析。①小鼠谷胱甘肽含量,谷胱甘肽过氧化物酶活性、谷胱甘肽过氧化物酶活性和丙二醛含量比值:糖尿病组低于正常对照组,虫草组、维生素E组高于糖尿病组,差异有显著性意义(P<0.05～0.001)。②丙二醛含量:糖尿病组高于正常对照组,虫草组、维生素E组低于糖尿病组(P<0.001)。③糖尿病组线粒体呼吸链复合体Ⅱ+Ⅲ电子传递与质子泵出总比值、净比值低于正常对照组,降低了24.76%,32.31%(P<0.001)。虫草组、维生素E组线粒体呼吸链复合体Ⅱ+Ⅲ电子传递与质子泵出总比值、净比值显著回升,高于糖尿病组(P<0.001)。结论:线粒体呼吸过程产生的自由基在糖尿病的肝损伤中可能具有重要的作用,其原因与电子漏增加、电子传递与质子泵出脱偶联有关;同时也证明了虫草提取液能有效的拮抗由糖尿病引起的肝线粒体的氧化损伤。AIM： To analyze the protection of traditional Chinese medicine extract of Cordyceps sinensis （CS） for diabetes-induced oxidative injuries in mice from the angle of mitochondrial respiratory chain. METHODS： This experiment was completed in State Key Laboratory of Biomembrane and Membrane Biotechnology of Institute of Zoology, Chinese Academy of Sciences from March 1998 to July 2000. A total of 32 male Kunming mice were divided into four groups, each containing 8 mice. Normal control group was absent by any treatment, and other mice were treated with alloxan to induce diabetic models. Mice of CS extract group merely took 1 mL CS extract orally, mice of Vitamin E group were offered by 4 mg/kg Vitamin E of subcutaneous injection, and mice of diabetes group were not treated with any protective medicine. We determined the activity of antioxidant enzyme in mitochondria with colorimetry. We also designed to determine the parameter of liver mitochondria about rate of electron transfer to proton pump of mitochondrial respiratory chain complex Ⅱ ＋ Ⅲ （H^＋/2e）. RESULTS： All 32 mice entered the result analysis. ①The content of glutathione （GSH）, activity of GSH peroxidase （GSH-Px）, and GSH-Px/ malonaldehyde （MDA） were all decreased obviously in diabetes group than in normal control group, also significantly decreased in diabetes group than in CS extract group and Vitamin E group （P 〈 0.05-0.01）.②MDA content was higher in diabetes group than in normal contral group, but lower in CS extract group and vitamin E group than in diabetes group （P 〈 0.001）.③ Compared with normal control group, the total and net H^＋/2e of mitochondrial respiratory chain complex Ⅱ ＋ Ⅲ decreased by 24.76% and 32.31% respectively in diabetes group （P 〈 0.001）. After breeding diabetic mice with extract of CS and Vitamin E respectively, it was found that＇each data recovered remarkably, which were higher than those of diabetes group （P 〈 0.001）. CONCLUSION： Free radical generated

关 键 词：线粒体 氧化磷酸化 糖尿病 冬虫夏草 

分 类 号：R587.1[医药卫生—内分泌]