督脉电针与NSCs移植联合应用对大鼠脊髓全横断损伤组织NT-3含量及受体表达的影响  

Effects of combination of Du meridian electroacupuncture and neural stem cells transplanted on the content of nerotrophine-3 and expression of nerotrophine-3 receptor in injured tissue of rat spinal cord transected completely

作　　者：陈雅云[1] 曾园山[1] 张伟[1] 陈玉玲[2] 陈穗君[1] 

机构地区：[1]中山大学中山医学院组织胚胎学教研室神经科学研究室 [2]中山大学附属一院针灸科

出　　处：《中国康复医学杂志》2006年第9期779-781,F0005,共4页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金(30472132);广东省中医药管理局科研基金(101139和303013)资助项目

摘　　要：目的:探讨督脉电针与神经干细胞(NSCs)联合应用对大鼠脊髓全横断损伤组织神经营养素-3(NT-3)含量及其受体表达的影响。方法:将30只成年大鼠分为对照14d组、督脉电针14d组(电针14d组)、神经干细胞移植14d组(NSCs14d组)、督脉电针+神经干细胞移植14d组(电针NSCs14d组)、神经干细胞移植30d组(NSCs30d组)和督脉电针+神经干细胞移植30d组(电针NSCs30d组)6组,所有动物均实施T10段脊髓全横断手术,其中电针组和电针NSCs组于术后5d进行电针治疗。结果:①电针NSCs14d组受损伤的脊髓组织含有较高水平的NT-3,其次是电针14d组和NSCs14d组,对照14d组受损伤的脊髓组织含有较低水平的NT-3。②NSCs30d组和电针NSCs30d组脊髓全横断处的移植神经干细胞均有TrkC表达。结论:督脉电针与神经干细胞移植联合应用能够明显增高大鼠脊髓全横断损伤处邻近组织的NT-3水平;在大鼠脊髓损伤处及邻近组织有些移植神经干细胞表达TrkC。Objective：To explore the effects of combination of Du meridian electroacupuncture and neural stem cells （NSCs） transplanted on the content of nerotrophine-3（NT-3） and expression of nerotrophine-3 receptor（tyrosine protein kinase C,TrkC） in injured tissue of rat spinal cord transected completely. Method：Thirty adult rats were divided into six groups： control 14d group,DU meridian electroacupuncture plus 14d group （EA 14d group）,NSCs transplanted 14d group （NSCs 14d group）,DU meridian electroacupuncture plus NSCs transplanted 14d group （EA＋ NSCs transplanted 14d group）,NSCs transplated 30d group （NSCs 30d group） and DU meridian electroacupuncture plus NSCs transplanted 30d group （EA＋NSCs transplanted 30d group）. T10 spinal cord segment of all animals were completely transected. The rats of EA 14d group,EA＋NSCs transplanted 14d group and EA＋NSCs transplanted 30d group were treated with DU electroacupuncture 5 days after operation. Result： ①The content of NT-3 at injured spinal cord tissue was highest in EA＋NSCs transplated 14d group, secondly at EA 14d group and NSCs 14d group. The content of NT-3 was lowest in control 14d group.②Transplanted neural stem cells at completely transected site of spinal cord expressed TrkC in NSCs 30d group and EA＋NSCs transplanted 30d group. Conclusion：Combination of Du meridian electroacupuncture and neural stem cells transplanted may significantly enhance NT-3 content of the tissue neighboring completely transected site of rat spinal cord. Some transplanted neural stem cells express TrkC in injured site and near tissue of rat spinal cord.

关 键 词：督脉电针 神经干细胞移植 脊髓损伤 神经营养素-3 神经营养素-3受体 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]