不同培养条件对薄荷试管苗玻璃化现象的影响  

Effects of different conditions of tissue culture on vitrification of Mentha haplocalyx Briq.plantlets

作　　者：王小敏[1] 李维林[2] 赵志强[3] 粱呈元[2] 房海灵[2] 

机构地区：[1]南京农业大学园艺学院,江苏南京210095 [2]江苏省.中国科学院植物研究所(南京中山植物园),江苏南京210014 [3]江苏省科学技术厅,江苏南京210008

出　　处：《植物资源与环境学报》2006年第3期51-54,共4页Journal of Plant Resources and Environment

基　　金：江苏省"十五"科技攻关项目(BE2002309);江苏省"十五"高技术研究项目(BG2005317)

摘　　要：以江苏东台产薄荷(M entha haplocalyxB riq.)的茎尖为外植体,以MS为基本培养基,研究了培养基添加物和培养条件对薄荷试管苗玻璃化现象的影响。结果显示,导致薄荷玻璃化苗产生的主要因素是培养基中的6-BA、蔗糖和琼脂浓度以及培养温度和光照时间;当6-BA浓度为2 mg.L-1、蔗糖浓度为4%、琼脂浓度为0.70%、培养温度25℃、光照12 h.d-1(2 000 lx)时,薄荷试管苗的繁殖系数较高,玻璃化苗率较低。The shoots of Mentha haplocalyx Briq. from Dongtai of Jiangsu Province were cultured in MS medium to observe the influence of additives and culture condition on vitrification of plantlets. The results showed that the main factors leading to vitrification of M. haplocalyx plantlets were concentrations of 6- BA, sucrose and agar in the culture medium, culture temperature and irradiation time. Under the condition of 2 mg · L^-1 6-BA, 4% sucrose, 0.70% agar and culture temperature 25℃, irradiation time 12 h ·d^-1 （2 000 lx）, the coefficient of proliferation is higher, the rate of vitrification is lower.

关 键 词：薄荷 组织培养 玻璃化 影响因素 

分 类 号：S567.235[农业科学—中草药栽培]