邻苯二甲酸二环己酯人工抗原的制备研究  

Study of dicyclohexyl phthalate on preparation and characterization of artificial antigen

作　　者：张明翠[1] 庄惠生[1] 郎庆[1] 

机构地区：[1]东华大学环境科学与工程学院,上海200051

出　　处：《卫生研究》2006年第5期543-546,共4页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.20277005)

摘　　要：目的合成邻苯二甲酸二环己酯半抗原衍生物和合格的人工全抗原,探讨其制备方法。方法通过保留环己基结构和在芳环上引入氨基取代基合成了一种邻苯二甲酸二环己酯半抗原衍生物4-氨基邻苯二甲酸二环己酯,产物经通过1HNMR、IR和UV确证了结构。半抗原衍生物再通过重氮化反应与BSA偶联。结果得到的半抗原衍生物4-硝基邻苯二甲酸二环己酯的2个紫外吸收峰分别为:λ1=214nm,λ2=256nm;4-氨基邻苯二甲酸二环己酯的2个紫外吸收峰分别为:λ1=226nm,λ2=288nm。人工全抗原4-氨基邻苯二甲酸二环己酯-BSA,经荧光光谱(λex=307nm,λem=468nm)、体系颜色变化和免疫实验确证偶联成功。抗原结合比为19∶1。人工全抗原通过免疫动物得到了效价高、特异性好、纯度也较高的抗体。结论通过保留环己基结构和在芳环上引入氨基的设计思路可以合成邻苯二甲酸二环己酯的人工全抗原。Objectlve To study the preparation and characterization of hapten and artificial antigen of dicyclohexyl phthalate. Methods A dicyelohexyl phthalate hapten （4-amino dieyclohexyl phthalate） was synthesized by introducing amino as a substituent on the aromatic ring and retaining the ester group, and characterized by I HNMR, IR and UV. The hapten was conjugated to BSA via amino diazotization linkage. Results λ1 = 214nm,λ2 = 256nm for the UV of dicyelohexyl 4-nitrophthalate and λ1= 226nm, λ2 = 288nm for the UV of dicyelohexyl 4-aminophthalate. Artificial antigen was prepared and tested by fluorescence, and λex = 307nm, λem ： 468nm, arid the approximate molar ratio of dieyclohexyl 4-aminophthalate to BSA waslg. The product was used as an immunogen, demonstrating that it is suitable for polyclonal antibody production. Conclusion It is a good method for preparation of artificial antigen of dicyclohexyl phthalate by introducing amino as a substituent on the aromatic ring and retaining the ester group. It was suggested that could supply excellent immune antigen for further preparation of antibody and immunoassay to dicyclohexyl phthalate.

关 键 词：邻苯二甲酸二环己酯 人工抗原 制备 表征 

分 类 号：X502[环境科学与工程—环境工程] Q503[生物学—生物化学]