云南热泉栖热菌属细菌(Thermussp)的质粒研究  

Studies on the Plasmids of Thermus Strains from Hot Sprimgs in Yunnan

作　　者：张加春[1] 张灼[1] 

机构地区：[1]云南大学生物系

出　　处：《云南大学学报（自然科学版）》1996年第2期95-98,共4页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省科委应用基础研究基金

摘　　要：从云南汤池、腾冲热泉（ｐＨ７．０～９．０，７０～９２℃）分离到８株专性嗜热非芽孢杆菌，最适生长温度６５～７５℃．鉴定为栖热菌属（Ｔｈｅｒｍｕｓ），根据生理生化特性测定，８株菌又分为两个群，分离自汤池样点的ＴＡ群５株菌氧化酶阳性，分离自腾冲样点的ＴＢ群３株菌，氧化酶阴性．通过质粒快速抽提，琼脂糖凝腋电脉发现，８株菌都含有一个质粒，大小略大于８．５ｋｂ．8 stranis of nonsporulating Thermophilic Bacteria Were isolated from hot springs (pH7.0~9.0 T:70~ 92℃) of Tangchi and Tengchong in Yunnan. They belong to Thermus which could be further devided into 2 groups: TA included 5 strsins isolsted from Tangchi which were oxidase-positive, TB included 3 strains from Tengchong which were oxidase-negative. By speed extraction and agarose gel eletrophoresis. It is easy to extract the Plasmids from the 8 strains, All the strains have single Pasmid. The Plasmids are about 8.5kb

关 键 词：栖热菌属 专性嗜热菌 质粒 热泉 细菌 

分 类 号：Q939.1[生物学—微生物学]