小鼠腹水IgG类单克隆抗体纯化方法的研究  

The study of purified methods of mice ascites IgG McAb

作　　者：周玉[1] 李岩松[2] 潘风光[2] 谭建华[3] 柳增善[1] 王哲[4] 

机构地区：[1]吉林大学人兽共患病研究所,吉林长春130062 [2]吉林大学军需科技学院,吉林长春130062 [3]军事医学科学院军事兽医研究所,吉林长春130062 [4]吉林大学农学部,吉林长春130062

出　　处：《黑龙江畜牧兽医》2006年第10期14-16,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然基金项目(30230620)

摘　　要：分别采用辛酸硫酸铵法(CA AS)、硫酸铵沉淀法(AS)和DEAE离子交换层析法对小鼠IgG腹水进行了纯化效果的比较。结果表明:CA AS法提取IgG的纯度为67.5%,高于AS法(43.2%),低于DEAE法(100%);CA AS法提取IgG的回收率为51.2%,远高于DEAE(8.9%),略低于AS法(63.8%)。将不同纯化方法获得的IgG定容至相同浓度。ELISA试验结果表明:DEAE法的OD值明显降低,表明DEAE纯化方法降低了IgG的免疫学活性。说明CA AS法是一种操作简单、成本低、纯化效果和回收效果较佳的提取小鼠腹水IgG的首选方法。Mice ascites lgG McAb was purified by caprylic/ammonium sulfate precipitation （CA -AS）, ammonium sulfate precipitation （AS） and ion- exchange chromatography （DEAE）. The purity of lgG from the method of CA - AS （67.5%） was higher than those from AS （43.2%） and lower than those from DEAE （ 100% ）. The recovery of lgG from CA - AS （51.2%） was higher than those from DEAE （8.9%） remarkably and lower than those from AS （63.8%） slightly. The concentration of purified lgG from different methods were adjusted the same as those of unpurified aseites. EL1SA shows that the OD values of DEAE method was lower remarkable, it indicated that the immunity of lgG was decreased by the purified method of DEAE. Conclusion： CA - AS s one simple, low - cost, and efficient method for purification of lgG from mice aseites

关 键 词：小鼠腹水IgG 辛酸 硫酸铵 离子交换层析 

分 类 号：S865.1[农业科学—野生动物驯养]