基于线粒体细胞色素b基因序列的蜻亚科部分种类分子系统学研究(蜻蜓目:蜻科)  

Phylogenetic Relationship of Some Species of Libellulinae Inferred from Sequences of Mitochondrial Cytochrome b Gene (Odonata:Libellulidae)

作　　者：张大治[1] 杨贵军[1] 郑哲民[2] 

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021 [2]陕西师范大学生命科学学院,陕西西安710062

出　　处：《宁夏大学学报（自然科学版）》2006年第3期255-259,共5页Journal of Ningxia University(Natural Science Edition)

基　　金：宁夏高等学校科研基金资助项目;宁夏大学自然科学基金资助项目(NS0511)

摘　　要：通过PCR及PCR产物的直接测序技术,测定了蜻蜓目蜻科蜻亚科的灰蜻属、宽腹蜻属和蜻属共7种蜻蜓的线粒体DNA细胞色素b基因的部分核苷酸序列(长度为576 bp),探讨了蜻亚科的分子系统学.在所有序列中A+T平均含量较高,为70.2%,而氨基酸密码子第3位点的A+T平均含量最高,为85.4%.多态位点数为180个,占总位点数的31.25%,显示出DNA序列变异丰富.基于邻接法、最大简约法和贝叶斯法的分子系统发生,分析结果支持灰蜻属是较进化的类群,宽腹蜻属次之,蜻属是相对分化较早、较古老的类群,这3个属的关系是蜻属→宽腹蜻属→灰蜻属.Partial nucleotide sequences of mitochondrial DNA cytochrome b gene from 7 species of 3 genera of Libellulinae in China were amplified by PCR and directly sequenced by silver-staining sequencing technique in this paper. The sequences were parts of Cyt b gene of mtDNA（576 base pairs in length）, and in the obtained sequences,（85.4%） than the other two sits. The sequence data revealed considerable variation in 180 nucleotide sites （about 31.25%） among the analyzed individuals from 3 genera, Orthetrum, Lyriothemis and Libellula. We obtained homologous nuclear sequence of Mnais maclachlani （damselfly） for outgroup. The phylogenetic trees were constructed with neighbor-joining method, maximum parsimony method and Bayesian inference. The confidence of nodes in the trees was evaluated by 1 000 replicates bootstrap. The phylogenetic relationship of mitochondrial DNA suggested that Orthetrum was more evolutional than Lyriothernis and Libellula. The phylogenetic relationships of the 3 genera was Libellula→L yriothernis→Orthetrum.

关 键 词：细胞色素b(Cyt 6) 线粒体DNA(mtDNA) 系统进化 蜻亚科 蜻科 蜻蜓目 

分 类 号：Q969.35[生物学—昆虫学]