雌二醇和孕酮对去势小鼠外周血白细胞和淋巴细胞的调节  

The regulation of estradiol and progesterone on leukocyte and lymphocyte of peripheral blood in ovariectomized mice

作　　者：时淑娟[1] 智建生[1] 任学军[2] 李云生[1] 

机构地区：[1]天津医科大学解剖学教研室,天津300070 [2]天津中医药大学基础医学实验中心

出　　处：《天津医科大学学报》2006年第3期378-380,共3页Journal of Tianjin Medical University

摘　　要：目的:探讨雌二醇、孕酮对去势小鼠外周血白细胞和淋巴细胞的调节。方法:雌性昆明小鼠60只随机分成5组,鼠龄35 d时除选取1组保留卵巢作为空白对照组不给予药物外,其余4组均去势,从鼠龄40 d开始注射药物,分别注射生理剂量的苯甲酸雌二醇、己酸羟孕酮或二者联合应用以及麻油,每5 d注射1次,共150 d。油镜下进行外周血白细胞及淋巴细胞计数。结果:空白对照组与苯甲酸雌二醇组相比,外周血白细胞数和淋巴细胞比例差异无统计学意义(P>0.05)。麻油组白细胞数和淋巴细胞比例均有明显下降,与其它4组比较差异均有统计学意义(P<0.05)。己酸羟孕酮组和苯甲酸雌二醇与己酸羟孕酮联合应用组白细胞数和淋巴细胞比例均增高(P<0.05),而二者之间差异无统计学意义。结论:苯甲酸雌二醇、己酸羟孕酮或二者联合应用均可以升高外周血白细胞数目和淋巴细胞数目。Objective： To investigate the regulation of estradiol and progesterone on leukocyte and lymphocyte of peripheral blood in ovariectomized mice. Methods： 60 kunming female mice were divided into 5 group：at random. 12 intact normal mice were as the control, 36 ovariectomized female mice were treated with EB, HPC, and the mixture of them at the normal dose respectively, and 12 ovariectomized female mice with sesame oil were as contrast. Injections of hormone were performed at 5-day intervals from 40 to 190 days of age, which were 30 times totally. Peripheral leukocytes and percentage of lymphocytes were counted. Results： There was no statistically significant, difference in the ratio of leukocytes and lymphocytes in the peripheral blood between INT group and OVX＋EB group （P〉0.05）. Compared with other four groups, there was a distinct drop of number of leukocytes and lymphocytes in OVX group （P〈0.05）. Although peripheral leukocytes and lymphocytes in HPC group and mixture of EB and HPC group showed a visible proliferation, there was no difference between them （P〉0.05）. Conclusion： Both estradiol benzoate and hydroxyprogesterone caproate or mixture of them can increase the count of peripheral leukocytes and percentage of lymphocytes.

关 键 词：小鼠 去势 苯甲酸雌二醇 己酸羟孕酮 白细胞 淋巴细胞 

分 类 号：R711.5[医药卫生—妇产科学] Q579.13[医药卫生—临床医学]