β-谷甾醇与马来酸酐酯化产物的表征及高效液相色谱分析  

Characterization and analysis by HPLC of the esterified product of β-sitosterol with maleic anhydride

作　　者：许文林[1] 王岩[1] 计明洁[1] 黄一波[1] 王雅琼[1] 

机构地区：[1]扬州大学化学化工学院,江苏扬州225002

出　　处：《扬州大学学报（自然科学版）》2006年第3期32-35,共4页Journal of Yangzhou University：Natural Science Edition

基　　金：国家自然科学基金资助项目(20576111);江苏省教育厅普通高等学校高新技术产业发展项目(JHB04033)

摘　　要：应用差示扫描量热法、红外光谱法、核磁共振波谱法和高效液相色谱法对β-谷甾醇与马来酸酐的酯化产物进行了表征和分析.实验结果表明:β-谷甾醇与马来酸酐发生酯化反应,产物为β-谷甾醇马来酸单酯;用Hypers il ODS反相柱,以甲醇为流动相,在检测波长为210 nm、柱温为25℃、流速为0.7 mL.m i-n 1的实验条件下进行液相色谱分析,β-谷甾醇及其马来酸单酯能够达到基线分离,采用外标法对β-谷甾醇及其马来酸单酯进行了定量分析,相对误差在±5%之间.外标法测得β-谷甾醇及其马来酸单酯的回收率接近100%.The esterified product of β-sitosterol with maleic anhydride was characterized and analyzed by DSC, IR, NMR and HPLC methods. The experimental results show that mono-β-sitosteryl maleate is the only esterified product of β-sitosterol with maleic anhydride. The β-sitiosterol and mono-β-sitosteryl maleate were analyzed using reverse-phase column Hypersil ODS with methanol as the mobile phase at 210 nm, 25 ℃ and flow rate 0.7 mL · min^-1. The β-sitosterol and the esterified product can be separated effectively and they can be quantitatively determined by the external standard method. Determined by the external standard method, the recoveries of β-sitosterol and mono-β-sitosteryl maleate were about 100% and the relative error was within ±5%.

关 键 词：Β-谷甾醇 β-谷甾醇马来酸单酯 表征 高效液相色谱法 

分 类 号：O655.4[理学—分析化学] O657.72[理学—化学]