肥料对秦艽(青海栽培)叶中龙胆苦苷含量的影响  

THE INFLUENCE OF MANURE ON GENTIOPICROSIDE'S CONTENT IN LEAVES OF QINGHAI PLANTING GENTIANA

作　　者：张华[1] 李福安[2] 李向阳[3] 李建民[4] 魏全嘉[2] 

机构地区：[1]青海大学医学院科技处 [2]青海大学医学院中医系 [3]青海大学医学院药学教研室 [4]青海师范大学生物系

出　　处：《青海医学院学报》2006年第3期189-191,共3页Journal of Qinghai Medical College

基　　金：国家科技攻关项目(2001BA701A60-06);青海省重大科技攻关项目(2001-N-107-03)

摘　　要：目的考察尿素-磷肥混合肥料对秦艽(青海栽培)叶中龙胆苦苷含量的影响。方法采用反相高效液相色谱法测定不同水平尿素-磷肥混合肥料施肥的秦艽叶中龙胆苦苷的含量。色谱柱为Lichrospher C18(4.6 mm×250 mm,5μm),流动相为甲醇-水(1∶2 v/v),检测波长为254 nm。结果不同水平的尿素-磷肥混合肥料对秦艽叶中龙胆苦苷的含量有影响。结论采用尿素-磷肥混合肥料(2025∶506比例)栽培秦艽其叶中龙胆苦苷含量较高。**Objective To investigate the influence of urea - phosphorus manure on Gentiopicroside＇ s content in leaves of Qinghai planting Gentiana. Methods The concentration of gentiopicroside was determined by RP - HPLC method in gentiana＇ s leaves planted by urea - phosphorus manure, the analysis involved a 250 mm × 4.6mm column packed with C 18 （5 μm）, the mobile phase consisted of methyl alcohol -water （ 1 ： 2）, and ultraviolet wavelength for detection is 254 nm. Results The effect on the level of spreading manure to Gentiopicroside＇ s content in planting gentiana＇ s leaves was significant, Conclusion The gentiopicroside of gentiana＇ s leaf planted with urea -phosphorus manure（2025：506 g/g） was more than other manure.

关 键 词：肥料 青海 栽培秦艽 龙胆苦苷 

分 类 号：Q949.776.4[生物学—植物学]