单子叶植物内生菌多糖对人红白血病K562细胞株抑制作用  

The inhabitation of K562 cell lines induced by a new polysaccharide produced from a new coniothyriunsp

作　　者：陈永乐[1] 乔治[2] 

机构地区：[1]武汉市第一医院病理科,湖北武汉430022 [2]武汉大学医学院病原生物学研究室,湖北武汉430030

出　　处：《中国医院药学杂志》2006年第10期1258-1261,共4页Chinese Journal of Hospital Pharmacy

摘　　要：目的:研究单子叶植物内生菌多糖对人红白血病K562细胞株的影响。方法:检测单子叶植物内生菌多糖对K562细胞剂量毒性和时间毒性作用,用凝胶电泳DNA梯度图谱定性分析肿瘤细胞凋亡,以及对K562细胞凋亡相关基因蛋白表达的影响。结果:单子叶植物内生菌多糖可诱导K562细胞产生凋亡;使K562细胞fax基因和bax基因表达增多(P<0.05或P<0.01);,bcl-2基因表达明显降低(P<0.01)。结论:单子叶植物内生菌多糖抗肿瘤活性的机制可能与引起肿瘤细胞凋亡密切相关。OBJECTIVE To investigate the function of the monocotyledon Coniothyriunsp polysaccharide to induce apoptosis of human Leucocythmia K562 cell lines in vitro. METHODS Gel electrophoresis DNA gradient diagram has been used to the apoptosis of the cells induced by the polysaccharide. RESULTS The phenomena of DNA＂Ladder＂,an apoptosis characteristic has been detected with agarose gel electrophoresis, the polysaccharide at different concentrations could reflected on the expression of bcl-2 increased, fax and bax decreased in the control group. CONCLUSION monocotyledon Coniothyriunsp polysaccharide is showed that apoptosis to K562 cell lines can been inducee by the polysaccharide.

关 键 词：单子叶植物内生菌 K562 细胞凋亡 

分 类 号：R965[医药卫生—药理学]