复方勒马回颗粒中麻黄碱含量测定  

HPLC determination of ephedrine in compound Lemahui granules

作　　者：李宏 于自勋 岳昌林 

机构地区：[1]浙江九旭药业有限公司,金华321016

出　　处：《药物分析杂志》2006年第9期1340-1342,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立复方勒马回颗粒中麻黄碱的含量测定方法。方法:样品用酸水研磨提取,采用HPLC法,色谱柱:Shimadzu CLC ODS(4.6mm×150mm,5μm),以0.1%磷酸(每1000mL含磷酸二氢钾3.4g)-甲醇-三乙胺(20:80:0.5)为流动相,流速1.0mL·min^(-1);检测波长为210nm。结果:线性范围为0.062～1.24μg,r=0.9998,平均回收率98.45%。结论:本方法能准确测定该制剂中麻黄碱的含量。Objective：To estabhsh an HPLC method ules. Method ：The chromatographic procedure carried for determination of ephedrine in compound Lemahui granout using a column of 150 mm long and 4.6 mm in internal diameter pacded with CLC ODS. As mobile phase, prepared a mixture of methanol - phosphoric acid （ with sodium dihydrogen phosphate 3.4 g per 1000 milliliter） - triethylamine（20： 80： 0. 5 ）. The flow rate was at 1.0 mL ·min ^-1 and the detective wavelength was at 210 nm. Results：The calibration curve of ephedrine hydrochloride was linear in the range of 0. 062 - 1.24μg with regression coefficient 0. 9998 . The average recoveries was 98.45 %. Conclusion：This method is accurate for the determination of ephedrine in compound Lemahui granule.

关 键 词：中药复方制剂 勒马回颗粒 麻黄碱 HPLC 

分 类 号：R917[医药卫生—药物分析学]