耐甲氧西林凝固酶阴性葡萄球菌mecA基因检测分析  

Detection and analysis of mecA gene of methicillin resistance coagulase negative slaphylococci

作　　者：金浩[1] 顾兵[2] 赵旺胜[2] 梅亚宁[2] 文怡[2] 张巧娣[2] 

机构地区：[1]盐城市第三人民医院检验科,江苏盐城224001 [2]南京医科大学第一附属医院临床检验中心,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2006年第10期906-908,共3页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:调查耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)耐药现状,分析其耐药表型与基因型的关系,为临床合理选用抗菌药物提供依据。方法:收集临床分离的86株凝固酶阴性葡萄球菌,采用头孢西丁纸片法检测耐药表型、聚合酶链反应(PCR)检测mecA基因,并将两种结果进行对比分析。结果:86株凝固酶阴性葡萄球菌中头孢西丁纸片法阳性64株,检出率为74.4%;mecA基因阳性58株,阳性率为67.4%;其中有1株头孢西丁纸片法阴性而mecA基因阳性,7株头孢西丁纸片法阳性而mecA基因阴性。结论:本地区耐甲氧西林凝固酶阴性葡萄球菌耐药状况严重;头孢西丁纸片法与PCR检测mecA基因一致率90.7%;PCR检测mecA基因可快速准确判定耐甲氧西林凝固酶阴性葡萄球菌。Objective： To investigate the antimicrobial resistance of methicillin resistance coagulase negative staphylococci （MRCNS）and to analyze the relationship between their phenotype and genotype. Methods： Eighty-six isolated strains of coagulase negative staphylococci （CNS）were collected to detect their drug resistance by cefoxitin disk diffusion method, mecA gene was detected by PCR. Results： Of the 86 strains of CNS, 64 strains showed resistance and 58 strains contained mecA gene. One strain was sensitivity to cefoxitin disk diffusion method but contained a meeA gene and 7 strains were resistant to oxacillin by cefoxitin disk diffusion method but showed negative in mecA gene. Conclusion： The rate of antibiotic resistance of MRCNS is high. By using PCR to detect meeA gene, MRCNS strains can be rapidly determined.

关 键 词：耐甲氧西林凝固酶阴性葡萄球菌 MECA基因 PCR 头孢西丁 耐药 

分 类 号：Q786[生物学—分子生物学]